• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

S1PR2 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 3.125-100 ng/mL

Sandwich ELISA: 1.56-100 ng/mL

Cat No : MP00831-1

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00831-1, Human S1PR2 Recombinant Matched Antibody Pair - PBS only. 83881-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag15470. 83881-1-PBS was HRP conjugated as the detection antibody. Range: 1.56-100 ng/mL.

    Sandwich ELISA standard curve of MP00831-1

    Sandwich ELISA standard curve of MP00831-1, Human S1PR2 Recombinant Matched Antibody Pair - PBS only. 83881-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag15470. 83881-1-PBS was HRP conjugated as the detection antibody. Range: 1.56-100 ng/mL.

  • The mean S1PR2 concentration was determined to be 53.98 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load and 33.91 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load.

    Sample test of MP00831-1

    The mean S1PR2 concentration was determined to be 53.98 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load and 33.91 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load.

  • Cytometric bead array standard curve of MP00831-1, S1PR2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83881-2-PBS. Detection antibody: 83881-1-PBS. Standard: Ag15470. Range: 3.125-100 ng/mL.

    Cytometric bead array standard curve of MP00831-1

    Cytometric bead array standard curve of MP00831-1, S1PR2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83881-2-PBS. Detection antibody: 83881-1-PBS. Standard: Ag15470. Range: 3.125-100 ng/mL.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00831-1 targets S1PR2 in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 3.125-100 ng/mL (Cytometric Bead Array); 1.56-100 ng/mL (Sandwich ELISA);

Capture antibody: S1PR2 Recombinant antibody, PBS Only (Capture) 83881-2-PBS (241067H8). 100 μg. Concentration 1 mgl/ml.

Detection antibody: S1PR2 Recombinant antibody, PBS Only (Detector) 83881-1-PBS (241067B10). 100 μg. Concentration 1 mgl/ml.

Matched pair protein standard: Ag15470

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83881-2-PBS
Tested Reactivity human
Immunogen S1PR2 fusion protein Ag15470
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name sphingosine-1-phosphate receptor 2
Synonyms Gpcr13, Endothelial differentiation G-protein coupled receptor 5, EDG5, EDG 5, AGR16
GenBank accession numberBC069598
Gene symbol S1PR2
UniprotO95136
Gene ID (NCBI) 9294
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83881-1-PBS
Tested Reactivity human
Immunogen S1PR2 fusion protein Ag15470
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name sphingosine-1-phosphate receptor 2
Synonyms Gpcr13, Endothelial differentiation G-protein coupled receptor 5, EDG5, EDG 5, AGR16
GenBank accession numberBC069598
Gene symbol S1PR2
UniprotO95136
Gene ID (NCBI) 9294
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.