Validation Data Gallery
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Tested Applications
| Positive WB detected in | PC-3 cells, Jurkat |
| Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IF | See 2 publications below |
Product Information
55404-1-AP targets PLIN4 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | KIAA1881 |
| Calculated molecular weight | 141 kDa |
| Observed molecular weight | 141 kDa |
| GenBank accession number | NM_001080400 |
| Gene Symbol | KIAA1881 |
| Gene ID (NCBI) | 729359 |
| RRID | AB_2881321 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96Q06 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PLIN4 (Perilipin 4) is a member of the perilipin family, a group of proteins that coat lipid droplets in adipocytes, the fatty tissue cells responsible for storing fat. The proteins in the Perilipin family are crucial regulators of lipid storage. PLIN4 expression is elevated in obese animals and humans. Plin4 is a lipid droplet protein (LDP) found predominantly in white adipose tissue (PMID: 23423172). It has lower expression in the heart, skeletal muscle, and brown adipose tissue (PMID: 17878492).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PLIN4 antibody 55404-1-AP | Download protocol |
| IHC protocol for PLIN4 antibody 55404-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Neurosci TDP-43 extracted from frontotemporal lobar degeneration subject brains displays distinct aggregate assemblies and neurotoxic effects reflecting disease progression rates. | ||
J Lipid Res Hormone-sensitive lipase protects adipose triglyceride lipase-deficient mice from lethal lipotoxic cardiomyopathy. | ||
Ann Clin Transl Neurol Subsarcolemmal and cytoplasmic p62 positivity and rimmed vacuoles are distinctive for PLIN4-myopathy | ||
Redox Biol Plin4 exacerbates cadmium-decreased testosterone level via inducing ferroptosis in testicular Leydig cells | ||
Biol Reprod NR4A1-mediated regulation of lipid droplets in progesterone synthesis in goat luteal cells† |