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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

S6 Ribosomal protein Monoclonal antibody, PBS Only

S6 Ribosomal protein Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C3E10

Cat no : 66886-1-PBS

Synonyms

RPS6, S6, RP S6, ribosomal protein S6, Phosphoprotein NP33

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using S6 Ribosomal protein Monoclonal antibody (66886-1-Ig)

    WB analysis using 66886-1-Ig (same clone as 66886-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66886-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using S6 Ribosomal protein Monoclonal antibody (66886-1-Ig)

    WB analysis of NIH/3T3 using 66886-1-Ig (same clone as 66886-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66886-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using S6 Ribosomal protein Monoclonal antibody (66886-1-Ig)

    WB analysis using 66886-1-Ig (same clone as 66886-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66886-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using S6 Ribosomal protein Monoclonal antibody (66886-1-Ig)

    WB analysis of rat liver using 66886-1-Ig (same clone as 66886-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66886-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using S6 Ribosomal protein Monoclonal antibody (66886-1-Ig)

    IHC staining of human placenta using 66886-1-Ig (same clone as 66886-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66886-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using S6 Ribosomal protein Monoclonal antibody (66886-1-Ig)

    IHC staining of human placenta using 66886-1-Ig (same clone as 66886-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66886-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using S6 Ribosomal protein Monoclonal antibody (66886-1-Ig)

    IHC staining of mouse pancreas using 66886-1-Ig (same clone as 66886-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66886-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat stomach tissue using S6 Ribosomal protein Monoclonal antibody (66886-1-Ig)

    IHC staining of rat stomach using 66886-1-Ig (same clone as 66886-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66886-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using S6 Ribosomal protein Monoclonal antibody (66886-1-Ig)

    IF Staining of HepG2 using 66886-1-Ig (same clone as 66886-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66886-1-Ig (S6 Ribosomal protein antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66886-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using S6 Ribosomal protein Monoclonal antibody (66886-1-Ig)

    FC experiment of HepG2 using 66886-1-Ig (same clone as 66886-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human S6 Ribosomal protein (66886-1-Ig, Clone:1C3E10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66886-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66886-1-PBS targets S6 Ribosomal protein in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen S6 Ribosomal protein fusion protein Ag6599 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S6
Calculated molecular weight 29 kDa
Observed molecular weight 29-32 kDa
GenBank accession numberBC000524
Gene symbol RPS6
Gene ID (NCBI) 6194
RRIDAB_2882218
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.