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KD/KO Validated

SACM1L Polyclonal antibody

Name: SACM1L Polyclonal antibody
Brand: Proteintech
SKU: 13033-1-AP

SACM1L Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13033-1-AP

Synonyms

Suppressor of actin mutations 1-like protein, SAC1, Phosphatidylinositol-4-phosphate phosphatase, Phosphatidylinositol-3-phosphatase SAC1, KIAA0851

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of human kidney using 13033-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 13033-1-AP (SACM1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 13033-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13033-1-AP (SACM1L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 13033-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13033-1-AP (SACM1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 13033-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 13033-1-AP (SACM1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 13033-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 13033-1-AP (SACM1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse kidney using 13033-1-AP
IP result of anti-SACM1L (IP:13033-1-AP, 4ug; Detection:13033-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.
IHC staining of human brain using 13033-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 13033-1-AP (SACM1L antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 13033-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 13033-1-AP (SACM1L antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human heart using 13033-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13033-1-AP (SACM1L Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 13033-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13033-1-AP (SACM1L Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of A549 using 13033-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 13033-1-AP (SACM1L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 13033-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	human kidney tissue, A549 cells, mouse kidney tissue, mouse lung tissue, human brain tissue
Positive IP detected in	mouse kidney tissue
Positive IHC detected in	human brain tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 15 publications below
IF	See 6 publications below
IP	See 1 publications below

Product Information

13033-1-AP targets SACM1L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SACM1L fusion protein Ag3682 相同性解析による交差性が予測される生物種
Full Name	SAC1 suppressor of actin mutations 1-like (yeast)
Calculated molecular weight	587 aa, 67 kDa
Observed molecular weight	60-67 kDa
GenBank accession number	BC016559
Gene symbol	SACM1L
Gene ID (NCBI)	22908
RRID	AB_2301284
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SACM1L(Suppressor of actin mutations 1-like protein) is also named KIAA0851. It is a phosphoinositide phosphatase that hydrolyzes PtdIns3P and PtdIns4P and has low activity towards PtdIns(3,5)P2.

Protocols

Product Specific Protocols
WB protocol for SACM1L antibody 13033-1-AP	Download protocol
IHC protocol for SACM1L antibody 13033-1-AP	Download protocol
IF protocol for SACM1L antibody 13033-1-AP	Download protocol
IP protocol for SACM1L antibody 13033-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB	Cell Res Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity Authors - Ying Gao View Article
	IP	Cell Host Microbe Global mapping of Salmonella enterica-host protein-protein interactions during infection. Authors - Philipp Walch View Article
mouse	WB	Neuron An ER Assembly Line of AMPA-Receptors Controls Excitatory Neurotransmission and Its Plasticity. Authors - Jochen Schwenk View Article
human	WB	Autophagy PtdIns4P restriction by hydrolase SAC1 decides specific fusion of autophagosomes with lysosomes. Authors - Hongjun Zhang KD Validated View Article
human	WB	Proc Natl Acad Sci U S A Addiction to Golgi-resident PI4P synthesis in chromosome 1q21.3-amplified lung adenocarcinoma cells. Authors - Lei Shi View Article
human	WB,IF	J Cell Biol Sensing of nutrients by CPT1C controls SAC1 activity to regulate AMPA receptor trafficking. Authors - Maria Casas View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SACM1L Polyclonal antibody

SACM1L Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of human kidney using 13033-1-AP

WB analysis using 13033-1-AP

WB analysis of mouse kidney using 13033-1-AP

WB analysis of mouse lung using 13033-1-AP

WB analysis of human brain using 13033-1-AP

IP experiment of mouse kidney using 13033-1-AP

IHC staining of human brain using 13033-1-AP

IHC staining of human brain using 13033-1-AP

IHC staining of human heart using 13033-1-AP

IHC staining of human heart using 13033-1-AP

IF Staining of A549 using 13033-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity

Global mapping of Salmonella enterica-host protein-protein interactions during infection.

An ER Assembly Line of AMPA-Receptors Controls Excitatory Neurotransmission and Its Plasticity.

PtdIns4P restriction by hydrolase SAC1 decides specific fusion of autophagosomes with lysosomes.

Addiction to Golgi-resident PI4P synthesis in chromosome 1q21.3-amplified lung adenocarcinoma cells.

Sensing of nutrients by CPT1C controls SAC1 activity to regulate AMPA receptor trafficking.