SAE1 Polyclonal antibody

Name: SAE1 Polyclonal antibody
Brand: Proteintech
SKU: 10229-1-AP

SAE1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10229-1-AP

Synonyms

SUMO-activating enzyme subunit 1, N-terminally processed, SUMO-activating enzyme subunit 1, SUA1, Sentrin/SUMO activating protein AOS1, HSPC140

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of A431 using 10229-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 10229-1-AP (SAE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10229-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10229-1-AP (SAE1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 10229-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 10229-1-AP (SAE1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10229-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10229-1-AP (SAE1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 10229-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 10229-1-AP (SAE1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 10229-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10229-1-AP (SAE1 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human colon cancer using 10229-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10229-1-AP (SAE1 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human skin cancer using 10229-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10229-1-AP (SAE1 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human skin cancer using 10229-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10229-1-AP (SAE1 Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of HeLa using 10229-1-AP
Immunofluorescent analysis of HeLa cells using 10229-1-AP (SAE1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 10229-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A431 cells, A549 cells, COLO 320 cells, HEK-293 cells, HeLa cells
Positive IHC detected in	human colon cancer tissue, human skin cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IHC	See 2 publications below

Product Information

10229-1-AP targets SAE1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SAE1 fusion protein Ag0275 相同性解析による交差性が予測される生物種
Full Name	SUMO1 activating enzyme subunit 1
Calculated molecular weight	38 kDa
Observed molecular weight	38 kDa
GenBank accession number	BC000344
Gene symbol	SAE1
Gene ID (NCBI)	10055
RRID	AB_2182917
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SAE1(SUMO-activating enzyme subunit 1) is also named as AOS1, SUA1, UBLE1A and belongs to the ubiquitin-activating E1 family. SAE1 has a calculated molecular mass of 38 kD. It had an apparent molecular mass of 40 kD by SDS-PAGE. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins(PMID:9920803). Western blot analysis of synchronized HeLa cells detectes increased AOS1 expression as cells progressed through S phase, followed by a substantial decrease in G2 phase. Immunofluorescence analysis shows AOS1 and UBA2 distributed throughout nuclei, but they are excluded from nucleoli.(PMID:11481243).

Protocols

Product Specific Protocols
WB protocol for SAE1 antibody 10229-1-AP	Download protocol
IHC protocol for SAE1 antibody 10229-1-AP	Download protocol
IF protocol for SAE1 antibody 10229-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Curr Biol Pumilio 1 suppresses multiple activators of p53 to safeguard spermatogenesis. Authors - Dong Chen View Article
human	IHC	Open Med (Wars) Bioinformatics-based analysis of SUMOylation-related genes in hepatocellular carcinoma reveals a role of upregulated SAE1 in promoting cell proliferation. Authors - Yang Liu View Article
human	WB	Nat Commun SENP1 prevents steatohepatitis by suppressing RIPK1-driven apoptosis and inflammation Authors - Lingjie Yan View Article
human	IHC,WB	Curr Issues Mol Biol SUMO-Activating Enzyme Subunit 1 Is Associated with Poor Prognosis, Tumor Progression, and Radio-Resistance in Colorectal Cancer Authors - Yueh-Jung Wu View Article
human	WB	Elife TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb. Authors - Xiancai Ma View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SAE1 Polyclonal antibody

SAE1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of A431 using 10229-1-AP

WB analysis of HeLa using 10229-1-AP

WB analysis of COLO 320 using 10229-1-AP

WB analysis of HEK-293 using 10229-1-AP

WB analysis of A549 using 10229-1-AP

IHC staining of human colon cancer using 10229-1-AP

IHC staining of human colon cancer using 10229-1-AP

IHC staining of human skin cancer using 10229-1-AP

IHC staining of human skin cancer using 10229-1-AP

IF Staining of HeLa using 10229-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Pumilio 1 suppresses multiple activators of p53 to safeguard spermatogenesis.

Bioinformatics-based analysis of SUMOylation-related genes in hepatocellular carcinoma reveals a role of upregulated SAE1 in promoting cell proliferation.

SENP1 prevents steatohepatitis by suppressing RIPK1-driven apoptosis and inflammation

SUMO-Activating Enzyme Subunit 1 Is Associated with Poor Prognosis, Tumor Progression, and Radio-Resistance in Colorectal Cancer

TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb.