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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SAE1 Recombinant antibody (84516-7-RR)

    WB analysis using 84516-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84516-7-RR (SAE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using SAE1 Recombinant antibody (84516-7-RR)

    FC experiment of HeLa using 84516-7-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SAE1 Recombinant antibody (84516-7-RR, Clone:241923C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84516-7-RR

    Affinity and Kinetic Characterization of 84516-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84516-7-RR against Human SAE1 were performed. The affinity constant is 1.98 nM.

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SAE1 Recombinant antibody

SAE1 Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84516-7-RR
Clone No.241923C6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

SUMO-activating enzyme subunit 1, SUA1, Sentrin/SUMO activating protein AOS1, HSPC140, AOS1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, A549 cells, A431 cells, HeLa cells, K-562 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84516-7-RR targets SAE1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SAE1 fusion protein Ag0275 相同性解析による交差性が予測される生物種
Full Name SUMO1 activating enzyme subunit 1
Calculated molecular weight 38 kDa
Observed molecular weight38 kDa
GenBank accession numberBC000344
Gene Symbol SAE1
Gene ID (NCBI) 10055
RRIDAB_3672023
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9UBE0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SAE1(SUMO-activating enzyme subunit 1) is also named as AOS1, SUA1, UBLE1A and belongs to the ubiquitin-activating E1 family. SAE1 has a calculated molecular mass of 38 kD. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins(PMID:9920803). Western blot analysis of synchronized HeLa cells detectes increased AOS1 expression as cells progressed through S phase, followed by a substantial decrease in G2 phase. Immunofluorescence analysis shows AOS1 and UBA2 distributed throughout nuclei, but they are excluded from nucleoli.(PMID:11481243).

Protocols

Product Specific Protocols
WB protocol for SAE1 antibody 84516-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols