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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse eye tissue using Retinal S antigen Polyclonal antibody (23546-1-AP)

    WB analysis of mouse eye using 23546-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 23546-1-AP (Retinal S antigen antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of rat eye tissue using Retinal S antigen Polyclonal antibody (23546-1-AP)

    IF Staining of rat eye using 23546-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat eye tissue using Retinal S antigen antibody (23546-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

Retinal S antigen Polyclonal antibody

Retinal S antigen Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 23546-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Synonyms

SAG, 48 kDa protein, Retinal S-antigen, Rod photoreceptor arrestin, S AG

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse eye tissue
Positive IF-P detected inrat eye tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23546-1-AP targets Retinal S antigen in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Retinal S antigen fusion protein Ag20318 相同性解析による交差性が予測される生物種
Full Name S-antigen; retina and pineal gland (arrestin)
Calculated molecular weight 405 aa, 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC156656
Gene Symbol Retinal S antigen
Gene ID (NCBI) 6295
RRIDAB_2879294
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10523
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Retinal S antigen antibody 23546-1-APDownload protocol
IF protocol for Retinal S antigen antibody 23546-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols