Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells, K-562 cells, U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68656-1-Ig targets RNF7 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | RNF7 fusion protein Ag16903 相同性解析による交差性が予測される生物種 |
| Full Name | ring finger protein 7 |
| Calculated molecular weight | 113 aa, 13 kDa |
| Observed molecular weight | 13 kDa |
| GenBank accession number | BC008627 |
| Gene Symbol | RNF7 |
| Gene ID (NCBI) | 9616 |
| RRID | AB_3670398 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9UBF6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RNF (ring finger protein 7 ) is a novel zinc RING finger protein which is redox responsive and protects mammalian cells from apoptosis.It is shown to be localised in the cytoplasmand nucleus of the cell and is expressed in the heart, liver, skeletal muscle predominantly. RNF7 forms oligomers(55 kDa),dimer(28 kDa) and monomer(13 kDa) in the presence of different concentrations of the reductant.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RNF7 antibody 68656-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |