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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SALL4 Recombinant antibody

SALL4 Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230284C4

Cat no : 83039-4-RR

Synonyms

Zinc finger protein 797, Sal-like protein 4, Sal like protein 4, HSAL4, DRRS

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using SALL4 Recombinant antibody (83039-4-RR)

    IF Staining of Caco-2 using 83039-4-RR

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using SALL4 antibody (83039-4-RR, Clone: 230284C4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using SALL4 Recombinant antibody (83039-4-RR)

    IF Staining of Caco-2 using 83039-4-RR

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using SALL4 antibody (83039-4-RR, Clone: 230284C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83039-4-RR

    Affinity and Kinetic Characterization of 83039-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83039-4-RR against Human SALL4 were performed. The affinity constant is 0.18 nM.

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Tested Applications

Positive IF/ICC detected inCaco-2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83039-4-RR targets SALL4 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SALL4 fusion protein Ag16076 相同性解析による交差性が予測される生物種
Full Name sal-like 4 (Drosophila)
Calculated molecular weight 1053 aa, 112 kDa
GenBank accession numberBC111714
Gene symbol SALL4
Gene ID (NCBI) 57167
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SALL4, also named Sal-like protein 4 or Zinc finger protein 797, Contains 7 C2H2-type zinc fingers and belongs to the sal C2H2-type zinc-finger protein family. SALL4 is constitutively expressed in acute myeloid leukemia. The constitutive expression of SALL4 in mice is sufficient to induce MDS-like symptoms and transformation to AML that is transplantable. SALL4 is able to bind beta-catenin and activate the Wnt/beta-catenin signaling pathway. Sequence analysis of the larger cDNA fragment isolated revealed a single, large open-reading frame, designated as SALL4A, that started from a strong consensus initiation sequence and was expected to encode 1053 amino acids. The other splicing variant of SALL4, designated SALL4B, lacked the region corresponding to amino acids 385 to 820 of the full-length SALL4A. The putative protein encoded by SALL4B cDNA was expected to consist of 617 amino acids.

Protocols

Product Specific Protocols
IF protocol for SALL4 antibody 83039-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols