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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SAMD14 Polyclonal antibody

SAMD14 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 22009-1-AP

Synonyms

Sterile alpha motif domain-containing protein 14, SAM domain-containing protein 14

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SAMD14 Polyclonal antibody (22009-1-AP)

    WB analysis using 22009-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22009-1-AP (SAMD14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SAMD14 Polyclonal antibody (22009-1-AP)

    IHC staining of human kidney using 22009-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22009-1-AP (SAMD14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SAMD14 Polyclonal antibody (22009-1-AP)

    IHC staining of human kidney using 22009-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22009-1-AP (SAMD14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SAMD14 Polyclonal antibody (22009-1-AP)

    IF Staining of HepG2 using 22009-1-AP

    Immunofluorescent analysis of HepG2 cells using 22009-1-AP (SAMD14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using SAMD14 Polyclonal antibody (22009-1-AP)

    FC experiment of HepG2 using 22009-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug SAMD14 Polyclonal antibody (22009-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inCOLO 320 cells, HEK-293 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22009-1-AP targets SAMD14 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SAMD14 fusion protein Ag15653 相同性解析による交差性が予測される生物種
Full Name sterile alpha motif domain containing 14
Calculated molecular weight 445 aa, 48 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC141822
Gene symbol SAMD14
Gene ID (NCBI) 201191
RRIDAB_2878969
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SAMD14 antibody 22009-1-APDownload protocol
IHC protocol for SAMD14 antibody 22009-1-APDownload protocol
IF protocol for SAMD14 antibody 22009-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols