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  • Featured Product
  • KD/KO Validated

SAMD4B Polyclonal antibody

SAMD4B Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17723-1-AP

Synonyms

hSmaug2, Protein Smaug homolog 2, SAMD4B, Smaug 2, SMAUG2, SMGB

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SAMD4B Polyclonal antibody (17723-1-AP)

    WB analysis of HeLa using 17723-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17723-1-AP (SAMD4B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SAMD4B Polyclonal antibody (17723-1-AP)

    WB analysis of mouse brain using 17723-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17723-1-AP (SAMD4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using SAMD4B Polyclonal antibody (17723-1-AP)

    WB analysis of mouse liver using 17723-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 17723-1-AP (SAMD4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using SAMD4B Polyclonal antibody (17723-1-AP)

    WB analysis of mouse kidney using 17723-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17723-1-AP (SAMD4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SAMD4B Polyclonal antibody (17723-1-AP)

    IF Staining of HepG2 using 17723-1-AP

    Immunofluorescent analysis of HepG2 cells, using SAMD4B antibody 17723-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, mouse kidney tissue, mouse liver tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17723-1-AP targets SAMD4B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SAMD4B fusion protein Ag12113 相同性解析による交差性が予測される生物種
Full Name sterile alpha motif domain containing 4B
Calculated molecular weight 694 aa, 75 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC054518
Gene symbol SAMD4B
Gene ID (NCBI) 55095
RRIDAB_2183381
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SAMD4B antibody 17723-1-APDownload protocol
IF protocol for SAMD4B antibody 17723-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Mol Immunol

SAMD4 family members suppress human hepatitis B virus by directly binding to the Smaug recognition region of viral RNA.

Authors - Yuze Wang
  • KO Validated
View Article
humanWB,IF

Mol Med Rep

miR‑451 suppresses the malignant characteristics of colorectal cancer via targeting SAMD4B.

Authors - Chunrong Wu
View Article