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  • Featured Product
  • KD/KO Validated

SAMD9 Polyclonal antibody

SAMD9 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22786-1-AP

Synonyms

OEF2, OEF1, NFTC, KIAA2004, DRIF1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SAMD9 Polyclonal antibody (22786-1-AP)

    WB analysis using 22786-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22786-1-AP (SAMD9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SAMD9 Polyclonal antibody (22786-1-AP)

    WB analysis of HEK-293 using 22786-1-AP

    WB result of SAMD9 antibody (22786-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SAMD9 transfected HEK-293 cells.

  • Western Blot (WB) analysis of HEK-293 cells using SAMD9 Polyclonal antibody (22786-1-AP)

    WB analysis of HEK-293 using 22786-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22786-1-AP (SAMD9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using SAMD9 Polyclonal antibody (22786-1-AP)

    IHC staining of human ovary cancer using 22786-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 22786-1-AP (SAMD9 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22786-1-AP targets SAMD9 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SAMD9 fusion protein Ag18787 相同性解析による交差性が予測される生物種
Full Name sterile alpha motif domain containing 9
Calculated molecular weight 1589 aa, 184 kDa
Observed molecular weight 148 kDa
GenBank accession numberBC132775
Gene symbol SAMD9
Gene ID (NCBI) 54809
RRIDAB_2879168
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sterile alpha motif domain-containing protein 9(SAMD9), aslo named as C7orf5, DRIF1, KIAA2004, OEF1, plays a role in growth factor signal transduction. SAMD9 is a ubiquitously expressed cytoplasmic protein encoded by two juxtaposed single-exon genes on chromosome 7q21(PMID: 34675305). Overexpression of SAMD9 protein in tissue culture results in reduction of cell proliferation rate and invasion index, and increasing activity of caspase 3(PMID: 25450373). An important paralog of this gene is SAMD9L. Observed MW of SAMD9 is from 148 kDa(PMID: 21248034).

Protocols

Product Specific Protocols
WB protocol for SAMD9 antibody 22786-1-APDownload protocol
IHC protocol for SAMD9 antibody 22786-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols