- Featured Product
- KD/KO Validated
SAMD9L Polyclonal antibody
SAMD9L Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 25173-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | rat kidney tissue, COLO 320 cells |
| Positive IHC detected in | human breast cancer tissue, mouse spleen tissue, human brain tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | COLO 320 cells, 3T3-L1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
25173-1-AP targets SAMD9L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SAMD9L fusion protein Ag18541 相同性解析による交差性が予測される生物種 |
| Full Name | sterile alpha motif domain containing 9-like |
| Calculated molecular weight | 1584 aa, 185 kDa |
| Observed molecular weight | 160-180 kDa |
| GenBank accession number | BC127118 |
| Gene symbol | SAMD9L |
| Gene ID (NCBI) | 219285 |
| RRID | AB_2879940 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Sterile alpha motif domain-containing protein 9-like(SAMD9L), also named as C7orf6, DRIF2, KIAA2005 and UEF, is a cytoplasmic protein that acts as a tumor suppressor but also plays a key role in cell proliferation and the innate immune response to viral infection. SAMD9L protein is an endosome fusion facilitator which causes myeloid malignancies in mice similar to human diseases with monosomy 7. The SAMD9L protein also appears to play an important role in the brain, particularly the part of the brain that coordinates movement (the cerebellum). SAMD9L has two isoforms with MW 185 kDa and 47 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SAMD9L antibody 25173-1-AP | Download protocol |
| IHC protocol for SAMD9L antibody 25173-1-AP | Download protocol |
| IF protocol for SAMD9L antibody 25173-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Med Clinical evolution, genetic landscape and trajectories of clonal hematopoiesis in SAMD9/SAMD9L syndromes. | ||
PLoS Pathog A paralogous pair of mammalian host restriction factors form a critical host barrier against poxvirus infection.
| ||
Immunobiology Shared biomarkers of multi-tissue origin for primary Sjogren's syndrome and their importance in immune microenvironment alterations | ||
Brain Commun New spinocerebellar ataxia subtype caused by SAMD9L mutation triggering mitochondrial dysregulation (SCA49). | ||
J Inflamm Res Integrated Analysis Identifies Upregulated SAMD9L as a Potential Biomarker Correlating with the Severity of Primary Sjögren's Syndrome |
