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  • KD/KO Validated

Sam50 Polyclonal antibody

Sam50 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20824-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

SAMM50, TOB55, SAMM 50, OMP85, CGI-51

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, A375 cells, mouse ovary tissue, mouse kidney tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20824-1-AP targets Sam50 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Sam50 fusion protein Ag14799 相同性解析による交差性が予測される生物種
Full Name sorting and assembly machinery component 50 homolog (S. cerevisiae)
Calculated molecular weight 469 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC007830
Gene Symbol Sam50
Gene ID (NCBI) 25813
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y512
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sam50, encoded by the SAMM50 gene, is an essential protein of the mitochondrial outer membrane (OMM). It contains a beta-barrel domain conserved from bacteria to man (PMID: 14570913). Sam50 is a part of the sorting and assembly machinery (SAM) necessary for the assembly of beta-barrel proteins in the OMM (PMID: 22252321). It is crucial for maintaining mitochondrial shape, morphology of mitochondrial cristae, and assembly of the mitochondrial respiratory chain complexes (PMID: 26587038; 22252321; 25781180). Polymorphisms in the SAMM50 gene have been associated with development and progression of nonalcoholic fatty liver disease (PMID: 23535911).

Protocols

Product Specific Protocols
WB protocol for Sam50 antibody 20824-1-APDownload protocol
IHC protocol for Sam50 antibody 20824-1-APDownload protocol
IF protocol for Sam50 antibody 20824-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Elife

QIL1 is a novel mitochondrial protein required for MICOS complex stability and cristae morphology.

Authors - Virginia Guarani
humanWB

Cell Rep

A Role for Mitochondrial Translation in Promotion of Viability in K-Ras Mutant Cells.

Authors - Timothy D Martin
  • KD Validated
mouseWB

Aging Cell

3D reconstruction of murine mitochondria reveals changes in structure during aging linked to the MICOS complex

Authors - Zer Vue
ratWB

Free Radic Biol Med

Regulation of mitochondrial cristae remodelling by acetylcholine alleviates palmitate-induced cardiomyocyte hypertrophy.

Authors - Run-Qing Xue
humanWB

J Cell Biol

The outer mitochondrial membrane protein TMEM11 demarcates spatially restricted BNIP3/BNIP3L-mediated mitophagy

Authors - Mehmet Oguz Gok
pigIF

Elife

Identification of candidate mitochondrial inheritance determinants using the mammalian cell-free system

Authors - Dalen Zuidema
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