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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SAP 97 Polyclonal antibody (29292-1-AP)

    WB analysis using 29292-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29292-1-AP (DLG1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SAP 97 Polyclonal antibody (29292-1-AP)

    WB analysis of HEK-293 using 29292-1-AP

    WB result of SAP 97 antibody (29292-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SAP 97 transfected HEK-293 cells.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SAP 97 Polyclonal antibody (29292-1-AP)

    IHC staining of human colon cancer using 29292-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29292-1-AP (DLG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SAP 97 Polyclonal antibody (29292-1-AP)

    IHC staining of human colon cancer using 29292-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29292-1-AP (DLG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SAP 97 Polyclonal antibody (29292-1-AP)

    IHC staining of human breast cancer using 29292-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29292-1-AP (DLG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SAP 97 Polyclonal antibody (29292-1-AP)

    IF Staining of HEK-293 using 29292-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SAP 97 antibody (29292-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

  • Featured Product
  • KD/KO Validated

SAP 97 Polyclonal antibody

SAP 97 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 29292-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

DLG1, Disks large homolog 1, SAP-97, SAP97, hdlg

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HUVEC cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29292-1-AP targets SAP 97 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SAP 97 fusion protein Ag31066 相同性解析による交差性が予測される生物種
Full Name discs, large homolog 1 (Drosophila)
Calculated molecular weight 100 kDa
Observed molecular weight100-130 kDa
GenBank accession numberNM_001098424
Gene Symbol SAP97
Gene ID (NCBI) 1739
RRIDAB_2918273
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12959
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DLG1, also naemd as SAP97, hDlg and DLGH1, belongs to the MAGUK family. It is essential multidomain scaffolding protein required for normal development. DLG1 may play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. It regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. DLG1 is a functional regulator of Kv1.5 channel. The DLG1-MPP7 complex promotes epithelial cell polarity and tight junction formation. The antibody is specific to DLG1.

Protocols

Product Specific Protocols
WB protocol for SAP 97 antibody 29292-1-APDownload protocol
IHC protocol for SAP 97 antibody 29292-1-APDownload protocol
IF protocol for SAP 97 antibody 29292-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols