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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SAP30 Polyclonal antibody

SAP30 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 27679-1-AP

Synonyms

SAP30

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of A549 cells using SAP30 Polyclonal antibody (27679-1-AP)

    WB analysis of A549 using 27679-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 27679-1-AP (SAP30 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using SAP30 Polyclonal antibody (27679-1-AP)

    WB analysis of K-562 using 27679-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 27679-1-AP (SAP30 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using SAP30 Polyclonal antibody (27679-1-AP)

    WB analysis of HL-60 using 27679-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 27679-1-AP (SAP30 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inA549 cells, HL-60 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27679-1-AP targets SAP30 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SAP30 fusion protein Ag26516 相同性解析による交差性が予測される生物種
Full Name Sin3A-associated protein, 30kDa
Calculated molecular weight 220 aa, 23 kDa
Observed molecular weight 23-28 kDa
GenBank accession numberBC016757
Gene symbol SAP30
Gene ID (NCBI) 8819
RRIDAB_2880945
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SAP30, also named as Sin3 corepressor complex subunit SAP30, is a 220 amino acid protein, which is expressed in all tissues tested with highest levels in pancreas, ovary, PBL, spleen and thymus. SAP30 is involved in the functional recruitment of the Sin3-histone deacetylase complex (HDAC) to a specific subset of N-CoR corepressor complexes.

Protocols

Product Specific Protocols
WB protocol for SAP30 antibody 27679-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Development

Novel dynamics of human mucociliary differentiation revealed by single-cell RNA sequencing of nasal epithelial cultures.

Authors - Sandra Ruiz García
View Article
humanWB

Cell Rep

SAP30BP interacts with RBM17/SPF45 to promote splicing in a subset of human short introns

Authors - Kazuhiro Fukumura
View Article