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  • Featured Product
  • KD/KO Validated

SAPS2 Polyclonal antibody

SAPS2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26190-1-AP

Synonyms

Serine/threonine-protein phosphatase 6 regulatory subunit 2, Serine/threonine protein phosphatase 6 regulatory subunit 2, SAPS domain family, member 2, SAPS domain family member 2, SAPS 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SAPS2 Polyclonal antibody (26190-1-AP)

    WB analysis using 26190-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26190-1-AP (SAPS2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SAPS2 Polyclonal antibody (26190-1-AP)

    WB analysis of HepG2 using 26190-1-AP

    WB result of SAPS2 antibody (26190-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SAPS2 transfected HepG2 cells.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using SAPS2 Polyclonal antibody (26190-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 26190-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26190-1-AP (SAPS2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using SAPS2 Polyclonal antibody (26190-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 26190-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26190-1-AP (SAPS2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26190-1-AP targets SAPS2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SAPS2 fusion protein Ag23458 相同性解析による交差性が予測される生物種
Full Name SAPS domain family, member 2
Calculated molecular weight 105 kDa
Observed molecular weight115-125 kDa
GenBank accession numberBC000976
Gene symbol SAPS2
Gene ID (NCBI) 9701
RRIDAB_3085847
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SAPS2 is a regulatory protein for the protein phosphatase-6 catalytic subunit. Together, these proteins act as a significant T-loop phosphatase for Aurora A, an essential mitotic kinase. Loss of function of either the regulatory or catalytic subunit of protein phosphatase-6 interferes with spindle formation and chromosome alignment.

Protocols

Product Specific Protocols
WB protocol for SAPS2 antibody 26190-1-APDownload protocol
IHC protocol for SAPS2 antibody 26190-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols