Featured Product
KD/KO Validated

SAR1A Polyclonal antibody

Name: SAR1A Polyclonal antibody
Brand: Proteintech
SKU: 22291-1-AP

SAR1A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 22291-1-AP

Synonyms

SARA, SAR1, GTP binding protein SAR1a, EC:3.6.5.2, COPII-associated small GTPase

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 22291-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22291-1-AP (SAR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 22291-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 22291-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 22291-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22291-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 22291-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 22291-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human skeletal muscle using 22291-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22291-1-AP (SAR1A Antibody) at dilution of 1:50.
IHC staining of mouse skeletal muscle using 22291-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22291-1-AP (SAR1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 22291-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SAR1A antibody (22291-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of A431 using 22291-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using SAR1A antibody (22291-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Cat No. 22291-1-AP

Size/Concentration

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A431 cells, HT-1080 cells, HepG2 cells, mouse kidney tissue, HEK-293T cells, HeLa cells
Positive IHC detected in	mouse skeletal muscle tissue, human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 5 publications below
CoIP	See 1 publications below

Product Information

22291-1-AP targets SAR1A in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	SAR1 homolog A (S. cerevisiae)
Calculated molecular weight	22 kDa
Observed molecular weight	23-25 kDa
GenBank accession number	NM_020150
Gene symbol	SAR1A
Gene ID (NCBI)	56681
RRID	AB_2879062
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SAR1A, also named as SAR1, SARA and SARA1, belongs to the small GTPase superfamily and SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus. SAR1A is required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES). The antibody is specific to SAR1A.

Protocols

Product Specific Protocols
WB protocol for SAR1A antibody 22291-1-AP	Download protocol
IHC protocol for SAR1A antibody 22291-1-AP	Download protocol
IF protocol for SAR1A antibody 22291-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature SAR1B senses leucine levels to regulate mTORC1 signalling. Authors - Jie Chen KO Validated View Article
human	CoIP	J Cell Sci A Wntless-SEC12 complex on the ER membrane regulates early Wnt secretory vesicle assembly and mature ligand export. Authors - Jiaxin Sun KD Validated View Article
rat	WB	Ann Transl Med Proteomics and metabolomics of raw rhubarb and wine-processed rhubarb in the treatment of rats with intracerebral hemorrhage. Authors - Kun Wang View Article
human	WB	Epigenomics Methylation of TMEM176A, a key ERK signaling regulator, is a novel synthetic lethality marker of ATM inhibitors in human lung cancer. Authors - Hongxia Li View Article
rat	WB	Cell Death Differ C9orf72 controls hepatic lipid metabolism by regulating SREBP1 transport Authors - Yachen Wu View Article
human	WB	J Cell Biol Small GTPase ActIvitY ANalyzing (SAIYAN) system: A method to detect GTPase activation in living cells Authors - Miharu Maeda View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SAR1A Polyclonal antibody

SAR1A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 22291-1-AP

WB analysis of HepG2 using 22291-1-AP

WB analysis of mouse kidney using 22291-1-AP

WB analysis of HT-1080 using 22291-1-AP

IHC staining of human skeletal muscle using 22291-1-AP

IHC staining of mouse skeletal muscle using 22291-1-AP

IF Staining of HepG2 using 22291-1-AP

IF Staining of A431 using 22291-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

SAR1B senses leucine levels to regulate mTORC1 signalling.

A Wntless-SEC12 complex on the ER membrane regulates early Wnt secretory vesicle assembly and mature ligand export.

Proteomics and metabolomics of raw rhubarb and wine-processed rhubarb in the treatment of rats with intracerebral hemorrhage.

Methylation of TMEM176A, a key ERK signaling regulator, is a novel synthetic lethality marker of ATM inhibitors in human lung cancer.

C9orf72 controls hepatic lipid metabolism by regulating SREBP1 transport

Small GTPase ActIvitY ANalyzing (SAIYAN) system: A method to detect GTPase activation in living cells