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Host
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SAR1A Recombinant antibody, PBS Only

SAR1A Recombinant Antibody for Indirect ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

230471A11

Cat no : 83168-6-PBS

Synonyms

SAR1, GTP binding protein SAR1a, EC:3.6.5.2, COPII-associated small GTPase, COPII associated small GTPase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SAR1A Recombinant antibody (83168-6-RR)

    WB analysis using 83168-6-RR (same clone as 83168-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83168-6-RR (SAR1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83168-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using SAR1A Recombinant antibody (83168-6-RR)

    FC experiment of A549 using 83168-6-RR (same clone as 83168-6-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug SAR1A Recombinant antibody (83168-6-RR, Clone:230471A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83168-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using SAR1A Recombinant antibody (83168-6-RR)

    FC experiment of A431 using 83168-6-RR (same clone as 83168-6-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug SAR1A Recombinant antibody (83168-6-RR, Clone:230471A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83168-6-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83168-6-PBS targets SAR1A in Indirect ELISA, WB applications and shows reactivity with mouse, human samples.

Tested Reactivity mouse, human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name SAR1 homolog A (S. cerevisiae)
Calculated molecular weight 22 kDa
GenBank accession numberNM_020150
Gene symbol SAR1A
Gene ID (NCBI) 56681
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.