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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SAR1B Polyclonal antibody

SAR1B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15453-1-AP

Synonyms

ANDD, CMRD, GTBPB, GTP binding protein B, GTP binding protein SAR1b, SAR1 homolog B (S. cerevisiae), SAR1B, SARA2, SARB

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SAR1B Polyclonal antibody (15453-1-AP)

    WB analysis using 15453-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15453-1-AP (SAR1B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using SAR1B Polyclonal antibody (15453-1-AP)

    WB analysis of human liver using 15453-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 15453-1-AP (SAR1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SAR1B Polyclonal antibody (15453-1-AP)

    WB analysis of mouse brain using 15453-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15453-1-AP (SAR1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SAR1B Polyclonal antibody (15453-1-AP)

    IHC staining of human stomach cancer using 15453-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15453-1-AP (SAR1B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SAR1B Polyclonal antibody (15453-1-AP)

    IHC staining of human stomach cancer using 15453-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15453-1-AP (SAR1B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inL02 cells, mouse brain tissue, human liver tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15453-1-AP targets SAR1B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SAR1B fusion protein Ag7718 相同性解析による交差性が予測される生物種
Full Name SAR1 homolog B (S. cerevisiae)
Calculated molecular weight 22 kDa
Observed molecular weight 25-27 kDa
GenBank accession numberBC002847
Gene symbol SAR1B
Gene ID (NCBI) 51128
RRIDAB_2183985
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SAR1B, also named as SARA2, SARB and GTBPB, belongs to the small GTPase superfamily and SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus. SAR1B is activated by the guanine nucleotide exchange factor PREB. It is involved in the selection of the protein cargo and the assembly of the COPII coat complex.

Protocols

Product Specific Protocols
WB protocol for SAR1B antibody 15453-1-APDownload protocol
IHC protocol for SAR1B antibody 15453-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols