Featured Product
KD/KO Validated

SAR1B Polyclonal antibody

Name: SAR1B Polyclonal antibody
Brand: Proteintech
SKU: 22292-1-AP

SAR1B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 22292-1-AP

Synonyms

ANDD, CMRD, GTBPB, GTP binding protein B, GTP binding protein SAR1b, SAR1 homolog B (S. cerevisiae), SAR1B, SARA2, SARB

Validation Data Gallery

Filter:

AllWBIHC

WB analysis of mouse liver using 22292-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 22292-1-AP (SAR1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse small intestine using 22292-1-AP
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 22292-1-AP (SAR1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 22292-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22292-1-AP (SAR1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of RAW264.7 using 22292-1-AP
RAW264.7 cells were subjected to SDS PAGE followed by western blot with 22292-1-AP (SAR1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 22292-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 22292-1-AP (SAR1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 22292-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 22292-1-AP (SAR1B Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.
IHC staining of human liver using 22292-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 22292-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human small intestine using 22292-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human small intestine using 22292-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skeletal muscle using 22292-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skeletal muscle using 22292-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 22292-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 22292-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skeletal muscle using 22292-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skeletal muscle using 22292-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22292-1-AP (SAR1B antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 22292-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse liver tissue, mouse brain tissue, mouse skeletal muscle tissue, mouse small intestine tissue
Positive IHC detected in	human liver tissue, human skeletal muscle tissue, human small intestine tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 7 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

22292-1-AP targets SAR1B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	SAR1 homolog B (S. cerevisiae)
Calculated molecular weight	22 kDa
Observed molecular weight	25-27 kDa
GenBank accession number	NM_016103
Gene symbol	SAR1B
Gene ID (NCBI)	51128
RRID	AB_11182713
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SAR1B, also named as SARA2, SARB and GTBPB, belongs to the small GTPase superfamily and SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus. SAR1B is activated by the guanine nucleotide exchange factor PREB. It is involved in the selection of the protein cargo and the assembly of the COPII coat complex. The antibody is specific to SAR1B.

Protocols

Product Specific Protocols
WB protocol for SAR1B antibody 22292-1-AP	Download protocol
IHC protocol for SAR1B antibody 22292-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IP	Nature SAR1B senses leucine levels to regulate mTORC1 signalling. Authors - Jie Chen KO Validated View Article
mouse	WB,IF	J Clin Invest Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling. Authors - Zhixiong Liu View Article
mouse,human	WB	Redox Biol Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis Authors - Mengyue You View Article
rat	WB	J Biol Chem The Endoplasmic Reticulum COPII-Vesicle Transport Machinery Coordinates Cellular Lipid Secretion and Cholesterol Biosynthesis. Authors - Lee G D Fryer View Article
mouse	WB	J Biol Chem Small intestine but not liver lysophosphatidylcholine acyltransferase 3 (Lpcat3) deficiency has a dominant effect on plasma lipid metabolism. Authors - Inamul Kabir View Article
rat	WB	Cell Death Differ C9orf72 controls hepatic lipid metabolism by regulating SREBP1 transport Authors - Yachen Wu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SAR1B Polyclonal antibody

SAR1B Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of mouse liver using 22292-1-AP

WB analysis of mouse small intestine using 22292-1-AP

WB analysis of mouse skeletal muscle using 22292-1-AP

WB analysis of RAW264.7 using 22292-1-AP

WB analysis of mouse brain using 22292-1-AP

WB analysis of mouse liver using 22292-1-AP

IHC staining of human liver using 22292-1-AP

IHC staining of human liver using 22292-1-AP

IHC staining of human small intestine using 22292-1-AP

IHC staining of human small intestine using 22292-1-AP

IHC staining of human skeletal muscle using 22292-1-AP

IHC staining of human skeletal muscle using 22292-1-AP

IHC staining of human liver using 22292-1-AP

IHC staining of human liver using 22292-1-AP

IHC staining of human skeletal muscle using 22292-1-AP

IHC staining of human skeletal muscle using 22292-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

SAR1B senses leucine levels to regulate mTORC1 signalling.

Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.

Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis

The Endoplasmic Reticulum COPII-Vesicle Transport Machinery Coordinates Cellular Lipid Secretion and Cholesterol Biosynthesis.

Small intestine but not liver lysophosphatidylcholine acyltransferase 3 (Lpcat3) deficiency has a dominant effect on plasma lipid metabolism.

C9orf72 controls hepatic lipid metabolism by regulating SREBP1 transport