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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SAR1B Monoclonal antibody

SAR1B Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B11A1

Cat no : 67765-1-Ig

Synonyms

ANDD, CMRD, GTBPB, GTP binding protein B, GTP binding protein SAR1b, SAR1 homolog B (S. cerevisiae), SAR1B, SARA2, SARB

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SAR1B Monoclonal antibody (67765-1-Ig)

    WB analysis using 67765-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67765-1-Ig (SAR1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SAR1B Monoclonal antibody (67765-1-Ig)

    WB analysis of HeLa using 67765-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67765-1-Ig (SAR1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SAR1B Monoclonal antibody (67765-1-Ig)

    WB analysis of HepG2 using 67765-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67765-1-Ig (SAR1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using SAR1B Monoclonal antibody (67765-1-Ig)

    WB analysis of L02 using 67765-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 67765-1-Ig (SAR1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inpig liver tissue, HeLa cells, HepG2 cells, L02 cells, mouse liver tissue, rat liver tissue, rabbit liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67765-1-Ig targets SAR1B in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Reactivity Human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SAR1B fusion protein Ag7718 相同性解析による交差性が予測される生物種
Full Name SAR1 homolog B (S. cerevisiae)
Calculated molecular weight 22 kDa
Observed molecular weight22-25 kDa
GenBank accession numberBC002847
Gene symbol SAR1B
Gene ID (NCBI) 51128
RRIDAB_2918532
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

SAR1B, also named as SARA2, SARB and GTBPB, belongs to the small GTPase superfamily and SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus. SAR1B is activated by the guanine nucleotide exchange factor PREB. It is involved in the selection of the protein cargo and the assembly of the COPII coat complex.

Protocols

Product Specific Protocols
WB protocol for SAR1B antibody 67765-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols