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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SARS-CoV-2 S protein (319-541 aa) Polyclonal antib (28991-1-AP)

    WB analysis using 28991-1-AP

    E.coli expressed SARS-CoV-2 spike protein (Cat.NO. Ag30688) was subjected to SDS PAGE followed by western blot with 28991-1-AP (SARS-CoV-2 S protein (319-541 aa) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • ELISA experiment of Recombinant protein using SARS-CoV-2 S protein (319-541 aa) Polyclonal antib (28991-1-AP)

    ELISA experiment of Recombinant protein using 28991-1-AP

    SARS-CoV-2 Spike Glycoprotein Antibody (28991-1-AP) tested by ELISA. SARS-CoV-2 Spike Glycoprotein (319-541 aa) were coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of SARS-CoV-2 Spike Glycoprotein Antibody (28991-1-AP). Bound antibodies were detected with HRP conjugated Goat anti-Rabbit IgG followed by incubation with HRP Substrate and teminated with H2SO4, then measuring the resulting absorbance at 450 nm.

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SARS-CoV-2 S protein (319-541 aa) Polyclonal antibody

SARS-CoV-2 S protein (319-541 aa) Polyclonal Antibody for WB, ELISA
Cat No. 28991-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Virus

Applications

WB, ELISA

Synonyms

SARS-CoV-2 Spike Glycoprotein (319-541 aa)

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRecombinant protein
Positive ELISA detected inRecombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28991-1-AP targets SARS-CoV-2 S protein (319-541 aa) in WB, ELISA applications and shows reactivity with Virus samples.

Tested Reactivity Virus
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SARS-CoV-2 S protein (319-541 aa) fusion protein Ag30689 相同性解析による交差性が予測される生物種
Full Name SARS-CoV-2 Spike Protein
Calculated molecular weight 141 kDa
GenBank accession numberNC_045512
Gene Symbol SARS-COV-2
Gene ID (NCBI) 43740568
RRIDAB_3086093
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616). 28991-1-AP is specific for spike protein of SARS-COV-2, that antigen region is 319-541aa.

Protocols

Product Specific Protocols
WB protocol for SARS-CoV-2 S protein (319-541 aa) antibody 28991-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols