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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SART1 Polyclonal antibody (22675-1-AP)

    WB analysis of HeLa using 22675-1-AP (same clone as 22675-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 22675-1-AP (SART1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22675-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using SART1 Polyclonal antibody (22675-1-AP)

    WB analysis of Jurkat using 22675-1-AP (same clone as 22675-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22675-1-AP (SART1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22675-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SART1 Polyclonal antibody (22675-1-AP)

    IP experiment of mouse brain using 22675-1-AP (same clone as 22675-1-PBS)

    IP result of anti-SART1 (IP:22675-1-AP, 4ug; Detection:22675-1-AP 1:6000) with mouse brain tissue lysate 2100 ug. This data was developed using the same antibody clone with 22675-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SART1 Polyclonal antibody (22675-1-AP)

    IHC staining of mouse testis using 22675-1-AP (same clone as 22675-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22675-1-AP (SART1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22675-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SART1 Polyclonal antibody (22675-1-AP)

    IHC staining of mouse testis using 22675-1-AP (same clone as 22675-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22675-1-AP (SART1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22675-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SART1 Polyclonal antibody (22675-1-AP)

    IF Staining of HepG2 using 22675-1-AP (same clone as 22675-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SART1 antibody (22675-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 22675-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

SART1 Polyclonal antibody, PBS Only

SART1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 22675-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

hSnu66, hSART-1, hSART 1, HOMS1, Hom s 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22675-1-PBS targets SART1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SART1 fusion protein Ag18478 相同性解析による交差性が予測される生物種
Full Name squamous cell carcinoma antigen recognized by T cells
Calculated molecular weight 800 aa, 90 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC001058
Gene Symbol SART1
Gene ID (NCBI) 9092
RRIDAB_2879148
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDO43290
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Squamous cell carcinoma antigen recognized by T cells (SART1), also known as U4/U6.U5 tri-snRNP-associated 110 kDa protein, is a ubiquitously expressed protein that is involved in mRNA splicing and the regulation of cell proliferation. It has been identified as regulator of c-FLIP and drug-induced activation of caspase 8. The SART1 gene encodes two proteins, the SART1(800) protein is located in the nucleus of the majority of proliferating cells, while the SART1(259) protein is located in the cytosol of epithelial cancers. Genetic variation in the SART1 gene may be associated with breast cancer development. This antibody recognizes the endogenous 800-amino acid SART1, which migrates with an apparent molecular mass of 110 kDa in SDS-PAGE. (PMID: 22027693; 9449708; 19377877; 11350945)