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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SASH1 Polyclonal antibody

SASH1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27689-1-AP

Synonyms

SH3D6A, SAM and SH3 domain-containing protein 1, RP3 323M4.1, Proline-glutamate repeat-containing protein, PEPE1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SASH1 Polyclonal antibody (27689-1-AP)

    WB analysis using 27689-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27689-1-AP (SASH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inSKOV-3 cells, U-251 cells, U-87 MG cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27689-1-AP targets SASH1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SASH1 fusion protein Ag26771 相同性解析による交差性が予測される生物種
Full Name SAM and SH3 domain containing 1
Calculated molecular weight 137 kDa
Observed molecular weight170 kDa
GenBank accession numberNM_015278
Gene symbol SASH1
Gene ID (NCBI) 23328
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SAM and SH3 domain-containing protein 1 (SASH1, also known as KIAA0790 and PEPE1) is a multidomain protein implicated in various cellular processes, including cell adhesion, migration, and apoptosis. It has been identified as a novel Eph receptor-binding partner through SAM-SAM domain interactions, which are critical for the recruitment and binding of downstream molecules in the Eph signaling pathway (PMID: 37619706). SASH1 is also known to regulate signaling through focal adhesion kinase (FAK) and AKT/PI3K and promote apoptosis. Mutations in SASH1 have been associated with cancers, and it is suggested as a potential therapeutic target in non-small cell lung cancer (PMID: 33122723).

Protocols

Product Specific Protocols
WB protocol for SASH1 antibody 27689-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols