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  • KD/KO Validated

SATB1 Polyclonal antibody

SATB1 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 15400-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, CoIP, ChIP, ELISA

Special AT-rich sequence-binding protein 1, DNA-binding protein SATB1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inhuman brain tissue, HEK-293 cells, mouse brain tissue, mouse spleen tissue, rat brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman oesophagus cancer tissue, human urothelial carcinoma tissue, mouse spleen tissue, mouse thymus tissue, rat brain tissue, rat spleen tissue, rat thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15400-1-AP targets SATB1 in WB, IHC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SATB1 fusion protein Ag7615 相同性解析による交差性が予測される生物種
Full Name SATB homeobox 1
Calculated molecular weight 86 kDa
Observed molecular weight 86-100 kDa
GenBank accession numberBC001744
Gene Symbol SATB1
Gene ID (NCBI) 6304
RRIDAB_2184321
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01826
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Epigenetic modifications and dynamic changes in chromatin organization by organizer proteins have recently been shown to play an instrumental role in regulating cancer-promoting genes. Special AT-rich binding protein (SATB1) is a unique type of global regulator that integrates higher-order chromatin organization -withregulation of gene expression. [PMID:23076250,22998183,23121661] SATB1 is a T cell-enriched transcription factor and a chromatin organizer essential for controlling genes that participate in T-cell development and activation. It regulates gene expression by periodically anchoring matrix attachment regions to the nuclear matrix and directly recruiting chromatin-modifying factors. Depending on its posttranslational modifications, SATB1 activates or represses multiple genes.Its expression is regulated by interleukin-4 (IL4) during T helper-2(Th2) cell differentiation[PMID: 20522714]. The calculated molecular weight of SATB1 is 86 kDa, but modified SATB1 is about 100 kDa (PMID: 22879953).

Protocols

Product Specific Protocols
WB protocol for SATB1 antibody 15400-1-APDownload protocol
IHC protocol for SATB1 antibody 15400-1-APDownload protocol
IP protocol for SATB1 antibody 15400-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Cell Rep

SATB1 regulates 3D genome architecture in T cells by constraining chromatin interactions surrounding CTCF-binding sites

Authors - Bao Wang
  • KO Validated
mouse,ratWB

Pharmacol Res

Cyclo-(Phe-Tyr) as a novel cyclic dipeptide compound alleviates ischemic/reperfusion brain injury via JUNB/JNK/NF-κB and SOX5/PI3K/AKT pathways.

Authors - Jiale Cai
mouseWB

Mol Neurobiol

SATB1/SLC7A11/HO-1 Axis Ameliorates Ferroptosis in Neuron Cells After Ischemic Stroke by Danhong Injection

Authors - Sikai Zhan
humanWB

Eur Rev Med Pharmacol Sci

Effect of STAT3 mediated epigenetic regulation in pre-eclampsia: an analysis of trial data.

Authors - X-L Zhang
humanWB

Int J Med Sci

SATB1 Knockdown Inhibits Proliferation and Invasion and Decreases Chemoradiation Resistance in Nasopharyngeal Carcinoma Cells by Reversing EMT and Suppressing MMP-9.

Authors - Dongni Zhou
  • KD Validated
humanWB

Int J Clin Exp Pathol

Silencing SATB1 influences cell invasion, migration, proliferation, and drug resistance in nasopharyngeal carcinoma.

Authors - Chun-Sheng Ye
  • KD Validated
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