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  • Featured Product
  • KD/KO Validated

SATB2 Monoclonal antibody, PBS Only (Capture)

SATB2 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F8E2

Cat no : 67958-1-PBS

Synonyms

KIAA1034, FLJ32076, FLJ21474, DNA-binding protein SATB2, DNA binding protein SATB2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using SATB2 Monoclonal antibody (67958-1-Ig)

    WB analysis using 67958-1-Ig (same clone as 67958-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67958-1-Ig (SATB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67958-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using SATB2 Monoclonal antibody (67958-1-Ig)

    WB analysis of NIH/3T3 using 67958-1-Ig (same clone as 67958-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67958-1-Ig (SATB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67958-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using SATB2 Monoclonal antibody (67958-1-Ig)

    WB analysis of HEK-293 using 67958-1-Ig (same clone as 67958-1-PBS)

    WB result of SATB2 antibody (67958-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SATB2 transfected HEK-293 cells. This data was developed using the same antibody clone with 67958-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using SATB2 Monoclonal antibody (67958-1-Ig)

    WB analysis of fetal human brain using 67958-1-Ig (same clone as 67958-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 67958-1-Ig (SATB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67958-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SW480 cells using SATB2 Monoclonal antibody (67958-1-Ig)

    WB analysis of SW480 using 67958-1-Ig (same clone as 67958-1-PBS)

    SW480 cells were subjected to SDS PAGE followed by western blot with 67958-1-Ig (SATB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67958-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SATB2 Monoclonal antibody (67958-1-Ig)

    WB analysis using 67958-1-Ig (same clone as 67958-1-PBS)

    HepG2 cell lysates and HepG2 nuclear protein were subjected to SDS PAGE followed by western blot with 67958-1-Ig (SATB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67958-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using SATB2 Monoclonal antibody (67958-1-Ig)

    IHC staining of human colon using 67958-1-Ig (same clone as 67958-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67958-1-Ig (SATB2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67958-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SATB2 Monoclonal antibody (67958-1-Ig)

    IHC staining of human colon cancer using 67958-1-Ig (same clone as 67958-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 67958-1-Ig (SATB2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67958-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using SATB2 Monoclonal antibody (67958-1-Ig)

    IHC staining of human colon using 67958-1-Ig (same clone as 67958-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67958-1-Ig (SATB2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67958-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50682-1

    Cytometric bead array standard curve of MP50682-1

    Cytometric bead array standard curve of MP50682-1, SATB2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67958-1-PBS. Detection antibody: 67958-2-PBS. Standard:Ag17237. Range: 0.781-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67958-1-PBS targets SATB2 as part of a matched antibody pair:

MP50682-1: 67958-1-PBS capture and 67958-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SATB2 fusion protein Ag17237 相同性解析による交差性が予測される生物種
Full Name SATB homeobox 2
Calculated molecular weight 733 aa, 83 kDa
Observed molecular weight85-100 kDa
GenBank accession numberBC098136
Gene symbol SATB2
Gene ID (NCBI) 23314
RRIDAB_2918709
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SATB2, also named as KIAA1034, belongs to the CUT homeobox family. SATB2 binds to DNA at nuclear matrix- or scaffold-associated regions. STAB2 recognizes the sugar-phosphate structure of double-stranded DNA. SATB2 is a transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. SATB2 acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. It is required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. It is a repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. SATB2 may play an important role in palate formation. SATB2 acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation. SATB2 has two isoforms produced by alternative splicing with the MW of 70 kDa and 83 kDa. It can be detected as 85-105 kDa by sumo modification (PMID: 14701874, PMID: 35140581).