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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SC65 Polyclonal antibody (15288-1-AP)

    WB analysis of HeLa using 15288-1-AP (same clone as 15288-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15288-1-AP (SC65 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15288-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SC65 Polyclonal antibody (15288-1-AP)

    WB analysis of HeLa using 15288-1-AP (same clone as 15288-1-PBS)

    WB result of SC65 antibody (15288-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SC65 transfected HeLa cells. This data was developed using the same antibody clone with 15288-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SC65 Polyclonal antibody (15288-1-AP)

    WB analysis using 15288-1-AP (same clone as 15288-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15288-1-AP (SC65 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15288-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse bladder tissue using SC65 Polyclonal antibody (15288-1-AP)

    IP experiment of mouse bladder using 15288-1-AP (same clone as 15288-1-PBS)

    IP result of anti-SC65 (IP:15288-1-AP, 4ug; Detection:15288-1-AP 1:500) with mouse bladder tissue lysate 3200ug. This data was developed using the same antibody clone with 15288-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

SC65 Polyclonal antibody, PBS Only

SC65 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 15288-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15288-1-PBS targets SC65 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SC65 fusion protein Ag7480 相同性解析による交差性が予測される生物種
Full Name synaptonemal complex protein SC65
Calculated molecular weight 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC001047
Gene Symbol SC65
Gene ID (NCBI) 10609
RRIDAB_2184611
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92791
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SC65, also named as Leprecan-like protein 4 or Nucleolar autoantigen No55, is a 437 amino acid protein, which belongs to the leprecan family. SC65 ia a protein of the interphase nucleolus and tumour-associated autoantigen in patients with prostate cancer.