Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, human liver tissue, MCF-7 cells, mouse pancreas tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human pancreas cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 10 publications below |
| IHC | See 4 publications below |
| IF | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
14799-1-AP targets RPS4X in WB, IHC, IF/ICC, IP, CoIP, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, xenopus, frog |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RPS4X fusion protein Ag6513 相同性解析による交差性が予測される生物種 |
| Full Name | ribosomal protein S4, X-linked |
| Calculated molecular weight | 263 aa, 30 kDa |
| Observed molecular weight | 30 kDa |
| GenBank accession number | BC000472 |
| Gene Symbol | RPS4X |
| Gene ID (NCBI) | 6191 |
| RRID | AB_2238567 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62701 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RPS4X, a X-linked gene in the human, encodes a subunit of ribosomal protein S4, a component of the 40S subunit and 60S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene, RPS4Y2 and the ribosomal protein S4, X-linked (RPS4X). [PMID:8358435]
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RPS4X antibody 14799-1-AP | Download protocol |
| IHC protocol for RPS4X antibody 14799-1-AP | Download protocol |
| IF protocol for RPS4X antibody 14799-1-AP | Download protocol |
| IP protocol for RPS4X antibody 14799-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Regnase-1 and Roquin Regulate a Common Element in Inflammatory mRNAs by Spatiotemporally Distinct Mechanisms. | ||
Nat Commun Excess ribosomal protein production unbalances translation in a model of Fragile X Syndrome. | ||
Elife Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons. | ||
Aging (Albany NY) Construction and external validation of a 5-gene random forest model to diagnose non-obstructive azoospermia based on the single-cell RNA sequencing of testicular tissue | ||
BMC Cancer Low expression of the X-linked ribosomal protein S4 in human serous epithelial ovarian cancer is associated with a poor prognosis.
| ||
Biomark Med Low level of the X-linked ribosomal protein S4 in human urothelial carcinomas is associated with a poor prognosis. |