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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SCARF1 Polyclonal antibody

SCARF1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28174-1-AP

Synonyms

Acetyl LDL receptor, KIAA0149, SCARF1, SREC, SREC-I

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SCARF1 Polyclonal antibody (28174-1-AP)

    WB analysis using 28174-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28174-1-AP (SCARF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHepG2 cells, SMMC-7721 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28174-1-AP targets SCARF1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCARF1 fusion protein Ag28079 相同性解析による交差性が予測される生物種
Full Name scavenger receptor class F, member 1
Calculated molecular weight 830 aa, 87 kDa
Observed molecular weight90 kDa, 180 kDa
GenBank accession numberBC039735
Gene symbol SCARF1
Gene ID (NCBI) 8578
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Scavenger receptor class F member 1 (SCARF1), also known as scavenger receptor expressed by endothelial cells (SREC)-I, belongs to the scavenger receptor (SR) superfamily. SCARF1 has been shown to bind modified low density lipoproteins (LDLs), including acetylated (Ac-) and oxidized (ox-) LDLs (PMID: 29725698; PMID: 15145948). In addition to binding and internalizing a diverse range of endogenous proteins, SCARF1 also binds a wide array of viral, fungal, and bacterial antigens (PMID: 29242513). SCARF1 has a calculated molecular weight of 87 kDa, with a molecular weight of 140-160 kDa due to N-glycosylation (PMID: 22279061; PMID: 15145948). SCARF1 can also exist as a dimer (~ 180 kDa) and a truncated soluble form (~ 60 kDa) (PMID: 29242513; PMID: 29725698).

Protocols

Product Specific Protocols
WB protocol for SCARF1 antibody 28174-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols