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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SCARF1 Polyclonal antibody

SCARF1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28174-1-AP

Synonyms

Acetyl LDL receptor, KIAA0149, SCARF1, SREC, SREC-I

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SCARF1 Polyclonal antibody (28174-1-AP)

    WB analysis using 28174-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28174-1-AP (SCARF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHepG2 cells, SMMC-7721 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28174-1-AP targets SCARF1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCARF1 fusion protein Ag28079 相同性解析による交差性が予測される生物種
Full Name scavenger receptor class F, member 1
Calculated molecular weight 830 aa, 87 kDa
Observed molecular weight90 kDa, 180 kDa
GenBank accession numberBC039735
Gene symbol SCARF1
Gene ID (NCBI) 8578
RRIDAB_3086035
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Scavenger receptor class F member 1 (SCARF1), also known as scavenger receptor expressed by endothelial cells (SREC)-I, belongs to the scavenger receptor (SR) superfamily. SCARF1 has been shown to bind modified low density lipoproteins (LDLs), including acetylated (Ac-) and oxidized (ox-) LDLs (PMID: 29725698; PMID: 15145948). In addition to binding and internalizing a diverse range of endogenous proteins, SCARF1 also binds a wide array of viral, fungal, and bacterial antigens (PMID: 29242513). SCARF1 has a calculated molecular weight of 87 kDa, with a molecular weight of 140-160 kDa due to N-glycosylation (PMID: 22279061; PMID: 15145948). SCARF1 can also exist as a dimer (~ 180 kDa) and a truncated soluble form (~ 60 kDa) (PMID: 29242513; PMID: 29725698).

Protocols

Product Specific Protocols
WB protocol for SCARF1 antibody 28174-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols