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  • Featured Product
  • KD/KO Validated

SCD Polyclonal antibody, PBS Only

SCD Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28678-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SCD Polyclonal antibody (28678-1-AP)

    WB analysis using 28678-1-AP (same clone as 28678-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28678-1-AP (SCD antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using SCD Polyclonal antibody (28678-1-AP)

    WB analysis of A431 using 28678-1-AP (same clone as 28678-1-PBS)

    WB result of SCD antibody (28678-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SCD transfected A431 cells. This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SCD Polyclonal antibody (28678-1-AP)

    IHC staining of human liver using 28678-1-AP (same clone as 28678-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SCD Polyclonal antibody (28678-1-AP)

    IHC staining of human liver using 28678-1-AP (same clone as 28678-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat heart tissue using SCD Polyclonal antibody (28678-1-AP)

    IHC staining of rat heart using 28678-1-AP (same clone as 28678-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brown adipose tissue using SCD Polyclonal antibody (28678-1-AP)

    IHC staining of mouse brown adipose using 28678-1-AP (same clone as 28678-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SCD Polyclonal antibody (28678-1-AP)

    IHC staining of mouse liver using 28678-1-AP (same clone as 28678-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SCD Polyclonal antibody (28678-1-AP)

    IHC staining of mouse liver using 28678-1-AP (same clone as 28678-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brown adipose tissue using SCD Polyclonal antibody (28678-1-AP)

    IHC staining of mouse brown adipose using 28678-1-AP (same clone as 28678-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SCD Polyclonal antibody (28678-1-AP)

    IHC staining of human colon cancer using 28678-1-AP (same clone as 28678-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using SCD Polyclonal antibody (28678-1-AP)

    IHC staining of human ovary tumor using 28678-1-AP (same clone as 28678-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SCD Polyclonal antibody (28678-1-AP)

    IHC staining of human stomach cancer using 28678-1-AP (same clone as 28678-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SCD Polyclonal antibody (28678-1-AP)

    IF Staining of MCF-7 using 28678-1-AP (same clone as 28678-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SCD antibody (28678-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28678-1-PBS targets SCD in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCD fusion protein Ag29156 相同性解析による交差性が予測される生物種
Full Name stearoyl-CoA desaturase (delta-9-desaturase)
Calculated molecular weight 355 aa, 41 kDa
Observed molecular weight28-42 kDa
GenBank accession numberBC005807
Gene symbol SCD
Gene ID (NCBI) 6319
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SCD1 (stearoyl-CoA desaturase) is a microsomal fatty acid monodesaturase, which catalyses the committed step in the biosynthesis of mono-unsaturated fatty acids from saturated fatty acids (PMID:10946019). SCD1 and SCD2 are the main isoforms expressed in mouse liver and brain respectively (PMID:15907797). The formation of homodimers and oligomers is an intrinsic property of SCD proteins. SCD1 is a multi-pass membrane protein and detected double bands of 37-42 kDa. The degradation product of 28 kDa may be caused by a major cleavage site at the C-terminus (PMID:15610069, PMID: 9843580).