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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SCD1 Recombinant antibody (81468-5-RR)

    WB analysis using 81468-5-RR (same clone as 81468-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81468-5-RR (SCD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81468-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SCD1 Recombinant antibody (81468-5-RR)

    IF Staining of MCF-7 using 81468-5-RR (same clone as 81468-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SCD antibody (81468-5-RR, Clone: 241487E12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81468-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using SCD1 Recombinant antibody (81468-5-RR)

    FC experiment of HepG2 using 81468-5-RR (same clone as 81468-5-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug SCD Recombinant antibody (81468-5-RR, Clone:241487E12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81468-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using SCD1 Recombinant antibody (81468-5-RR)

    FC experiment of MCF-7 using 81468-5-RR (same clone as 81468-5-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug SCD Recombinant antibody (81468-5-RR, Clone:241487E12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81468-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81468-5-RR

    Affinity and Kinetic Characterization of 81468-5-RR

    Biolayer interferometry (BLl) kinetic assays of 81468-5-RR against Human SCD were performed. The affinity constant is 1.83 nM.

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SCD1 Recombinant antibody, PBS Only

SCD1 Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 81468-5-PBS
Clone No.241487E12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

SCD, Delta-9 desaturase, Delta(9)-desaturase, Delta(9) desaturase, Acyl-CoA desaturase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81468-5-PBS targets SCD1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SCD1 fusion protein Ag29156 相同性解析による交差性が予測される生物種
Full Name stearoyl-CoA desaturase (delta-9-desaturase)
Calculated molecular weight 355 aa, 41 kDa
Observed molecular weight28-42 kDa
GenBank accession numberBC005807
Gene Symbol SCD
Gene ID (NCBI) 6319
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO00767
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SCD1 (stearoyl-CoA desaturase) is a microsomal fatty acid monodesaturase, which catalyses the committed step in the biosynthesis of mono-unsaturated fatty acids from saturated fatty acids (PMID:10946019). SCD1 and SCD2 are the main isoforms expressed in mouse liver and brain respectively (PMID:15907797). The formation of homodimers and oligomers is an intrinsic property of SCD proteins. SCD1 is a multi-pass membrane protein and detected double bands of 37-42 kDa. The degradation product of 28 kDa may be caused by a major cleavage site at the C-terminus (PMID:15610069, PMID: 9843580).