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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SCF Polyclonal antibody

SCF Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23844-1-AP

Synonyms

KITLG, KL 1, Kitl, KIT ligand, c-Kit ligand

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SCF Polyclonal antibody (23844-1-AP)

    WB analysis using 23844-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23844-1-AP (SCF antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SCF Polyclonal antibody (23844-1-AP)

    IF Staining of HEK-293 using 23844-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SCF antibody (23844-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SCF Polyclonal antibody (23844-1-AP)

    IF Staining of HepG2 using 23844-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SCF antibody (23844-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inK-562 cells
Positive IF/ICC detected inHepG2 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23844-1-AP targets SCF in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCF fusion protein Ag20837 相同性解析による交差性が予測される生物種
Full Name KIT ligand
Calculated molecular weight 273 aa, 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC074725
Gene symbol SCF
Gene ID (NCBI) 4254
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Stem cell factor (SCF) is also named as mast cell growth factor (MGF), Kit ligand (Kitl), c-Kit ligand, steel factor and Kitlg. SCF is a pleiotropic cytokine (also as a dimeric molecule) that mediates regulatory effects on inflammation, tissue remodeling, and fibrosis through binding to and activating its receptor tyrosine kinase c-Kit (PMID: 1698553, PMID: 8630386, PMID: 23073628). SCF is the ligand of c-Kit, a member of the RTKs family. Molecular activity of RTKs is a primary stimulus of cell motility, and expression of the SCF/c-Kit axis is associated with cell migration (PMID: 28629522). SCF is a cytokine that synergizes the effects of Epo during erythropoiesis. Stem cell factor plays a well-described role in regulating the survival, proliferation, migration, and differentiation of hematopoietic progenitors, melanocytes, and germ cells (PMID: 36688460). More recent studies have demonstrated SCF expression in dermal and intestinal epithelial cells, and increased SCF expression has been reported in the inflamed mucosa of individuals with IBD and colonitis (PMID: 18200049, PMID: 30157426).

Protocols

Product Specific Protocols
WB protocol for SCF antibody 23844-1-APDownload protocol
IF protocol for SCF antibody 23844-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols