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- KD/KO Validated
SCFD1 Polyclonal antibody
SCFD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 12569-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HEK-293T cells, human kidney tissue, human liver tissue, HEK-293 cells, HeLa cells, NIH/3T3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human endometrial cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 6 publications below |
| IP | See 1 publications below |
Product Information
12569-1-AP targets SCFD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, bacteria |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SCFD1 fusion protein Ag3234 相同性解析による交差性が予測される生物種 |
| Full Name | sec1 family domain containing 1 |
| Calculated molecular weight | 642 aa, 72 kDa |
| Observed molecular weight | 66-72 kDa |
| GenBank accession number | BC017734 |
| Gene symbol | SCFD1 |
| Gene ID (NCBI) | 23256 |
| RRID | AB_2183266 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SCFD1, also named as C14orf163, KIAA0917, STXBP1L2, Sly1p and SLY1, belongs to the STXBP/unc-18/SEC1 family. It is a component of the syntaxin-5 SNARE complex which regulates the early secretory pathway of eukaryotic cells at the level of endoplasmic reticulum (ER) to Golgi transport.(PMID:21242315) SCFD1 plays a role in SNARE-pin assembly and Golgi-to-ER retrograde transport via its interaction with COG4. It is involved in vesicular transport between the endoplasmic reticulum and the Golgi.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SCFD1 antibody 12569-1-AP | Download protocol |
| IHC protocol for SCFD1 antibody 12569-1-AP | Download protocol |
| IF protocol for SCFD1 antibody 12569-1-AP | Download protocol |
| IP protocol for SCFD1 antibody 12569-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO J Direct interaction between the COG complex and the SM protein, Sly1, is required for Golgi SNARE pairing. | ||
Autophagy Acetylation of SCFD1 regulates SNARE complex formation and autophagosome-lysosome fusion.
| ||
J Cell Sci Autophagic substrate clearance requires activity of the syntaxin-5 SNARE complex.
| ||
Microb Pathog Formononetin alleviates Streptococcus suis infection by targeting suilysin. | ||
J Appl Microbiol Silibinin attenuates Streptococcus suis serotype 2 virulence by targeting suilysin. | ||
Mol Biol Cell Recruitment of PI4KIIIβ to the Golgi by ACBD3 is dependent on an upstream pathway of a SNARE complex and golgins
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