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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SCFD2 Polyclonal antibody

SCFD2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13375-1-AP

Synonyms

Syntaxin-binding protein 1-like 1, STXBP1L1, Sec1 family domain-containing protein 2

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using SCFD2 Polyclonal antibody (13375-1-AP)

    WB analysis of COLO 320 using 13375-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 13375-1-AP (SCFD2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using SCFD2 Polyclonal antibody (13375-1-AP)

    WB analysis of COLO 320 using 13375-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 13375-1-AP (SCFD2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using SCFD2 Polyclonal antibody (13375-1-AP)

    WB analysis of mouse kidney using 13375-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13375-1-AP (SCFD2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of COLO 320 cells using SCFD2 Polyclonal antibody (13375-1-AP)

    IP experiment of COLO 320 using 13375-1-AP

    IP result of anti-SCFD2 (IP:13375-1-AP, 4ug; Detection:13375-1-AP 1:200) with COLO 320 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SCFD2 Polyclonal antibody (13375-1-AP)

    IHC staining of human colon cancer using 13375-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13375-1-AP (SCFD2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SCFD2 Polyclonal antibody (13375-1-AP)

    IHC staining of human colon cancer using 13375-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13375-1-AP (SCFD2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SCFD2 Polyclonal antibody (13375-1-AP)

    IHC staining of human colon cancer using 13375-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13375-1-AP (SCFD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using SCFD2 Polyclonal antibody (13375-1-AP)

    IHC staining of human colon using 13375-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13375-1-AP (SCFD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using SCFD2 Polyclonal antibody (13375-1-AP)

    IHC staining of human colon using 13375-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13375-1-AP (SCFD2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using SCFD2 Polyclonal antibody (13375-1-AP)

    IHC staining of human colon using 13375-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13375-1-AP (SCFD2 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inCOLO 320 cells, mouse kidney tissue
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman colon cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13375-1-AP targets SCFD2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCFD2 fusion protein Ag4197 相同性解析による交差性が予測される生物種
Full Name sec1 family domain containing 2
Calculated molecular weight 684 aa, 75 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC032453
Gene symbol SCFD2
Gene ID (NCBI) 152579
RRIDAB_10646473
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SCFD2 antibody 13375-1-APDownload protocol
IHC protocol for SCFD2 antibody 13375-1-APDownload protocol
IP protocol for SCFD2 antibody 13375-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols