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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Secretogranin V Polyclonal antibody

Secretogranin V Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10761-1-AP

Synonyms

SCG5, Secretogranin-5, N-terminal peptide, Neuroendocrine protein 7B2, C-terminal peptide

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using Secretogranin V Polyclonal antibody (10761-1-AP)

    WB analysis of mouse brain using 10761-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10761-1-AP (Secretogranin V antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat pancreas tissue using Secretogranin V Polyclonal antibody (10761-1-AP)

    IHC staining of rat pancreas using 10761-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 10761-1-AP (Secretogranin V antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat pancreas tissue using Secretogranin V Polyclonal antibody (10761-1-AP)

    IHC staining of rat pancreas using 10761-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 10761-1-AP (Secretogranin V antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pituitary adenoma tissue using Secretogranin V Polyclonal antibody (10761-1-AP)

    IHC staining of human pituitary adenoma using 10761-1-AP

    Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 10761-1-AP (Secretogranin V antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pituitary adenoma tissue using Secretogranin V Polyclonal antibody (10761-1-AP)

    IHC staining of human pituitary adenoma using 10761-1-AP

    Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 10761-1-AP (Secretogranin V antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Secretogranin V Polyclonal antibody (10761-1-AP)

    IHC staining of mouse pancreas using 10761-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10761-1-AP (Secretogranin V antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Secretogranin V Polyclonal antibody (10761-1-AP)

    IHC staining of mouse pancreas using 10761-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10761-1-AP (Secretogranin V antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using Secretogranin V Polyclonal antibody (10761-1-AP)

    IF Staining of PC-12 using 10761-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed PC-12 cells using 10761-1-AP (Secretogranin V antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IHC detected inrat pancreas tissue, human pituitary adenoma tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-12 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10761-1-AP targets Secretogranin V in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Secretogranin V fusion protein Ag1107 相同性解析による交差性が予測される生物種
Full Name secretogranin V (7B2 protein)
Calculated molecular weight 24 kDa
GenBank accession numberBC005349
Gene symbol Secretogranin V
Gene ID (NCBI) 6447
RRIDAB_2183280
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Secretogranin V antibody 10761-1-APDownload protocol
IHC protocol for Secretogranin V antibody 10761-1-APDownload protocol
IF protocol for Secretogranin V antibody 10761-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

Bromodomain protein BRD4 inhibitor JQ1 regulates potential prognostic molecules in advanced renal cell carcinoma.

Authors - Takashi Sakaguchi
View Article
mouseWB

Aging (Albany NY)

Plumbagin has an inhibitory effect on the growth of TSCC PDX model and it enhances the anticancer efficacy of cisplatin

Authors - Yuqi Xin
View Article
humanWB,IHC

Dis Esophagus

Expression profile and function of secretogranin V, and its effects on the malignant behavior of esophageal squamous cell carcinoma

Authors - Mohammad Hussain Hamrah
  • KD Validated
View Article