Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of mouse brain using 10761-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10761-1-AP (Secretogranin V antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of rat pancreas using 10761-1-AP
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 10761-1-AP (Secretogranin V antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat pancreas using 10761-1-AP
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 10761-1-AP (Secretogranin V antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pituitary adenoma using 10761-1-AP
Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 10761-1-AP (Secretogranin V antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pituitary adenoma using 10761-1-AP
Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 10761-1-AP (Secretogranin V antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse pancreas using 10761-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10761-1-AP (Secretogranin V antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse pancreas using 10761-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10761-1-AP (Secretogranin V antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of PC-12 using 10761-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed PC-12 cells using 10761-1-AP (Secretogranin V antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Secretogranin V Polyclonal antibody

Secretogranin V Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 10761-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SCG5, Secretogranin-5, N-terminal peptide, Neuroendocrine protein 7B2, C-terminal peptide

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive IHC detected in	rat pancreas tissue, human pituitary adenoma tissue, mouse pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	PC-12 cells

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

10761-1-AP targets Secretogranin V in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Secretogranin V fusion protein Ag1107 相同性解析による交差性が予測される生物種
Full Name	secretogranin V (7B2 protein)
Calculated molecular weight	24 kDa
GenBank accession number	BC005349
Gene Symbol	Secretogranin V
Gene ID (NCBI)	6447
RRID	AB_2183280
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P05408
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for Secretogranin V antibody 10761-1-AP	Download protocol
IHC protocol for Secretogranin V antibody 10761-1-AP	Download protocol
IF protocol for Secretogranin V antibody 10761-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Oncotarget Bromodomain protein BRD4 inhibitor JQ1 regulates potential prognostic molecules in advanced renal cell carcinoma. Authors - Takashi Sakaguchi View Article
mouse	WB	Aging (Albany NY) Plumbagin has an inhibitory effect on the growth of TSCC PDX model and it enhances the anticancer efficacy of cisplatin Authors - Yuqi Xin View Article
human	WB,IHC	Dis Esophagus Expression profile and function of secretogranin V, and its effects on the malignant behavior of esophageal squamous cell carcinoma Authors - Mohammad Hussain Hamrah KD Validated View Article
mouse	IF	Diabetologia Transcriptomic heterogeneity of non-beta islet cells is associated with type 2 diabetes development in mouse models Authors - Pascal Gottmann View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse brain using 10761-1-AP

IHC staining of rat pancreas using 10761-1-AP

IHC staining of rat pancreas using 10761-1-AP

IHC staining of human pituitary adenoma using 10761-1-AP

IHC staining of human pituitary adenoma using 10761-1-AP

IHC staining of mouse pancreas using 10761-1-AP

IHC staining of mouse pancreas using 10761-1-AP

IF Staining of PC-12 using 10761-1-AP

Secretogranin V Polyclonal antibody

Secretogranin V Polyclonal Antibody for IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Bromodomain protein BRD4 inhibitor JQ1 regulates potential prognostic molecules in advanced renal cell carcinoma.

Plumbagin has an inhibitory effect on the growth of TSCC PDX model and it enhances the anticancer efficacy of cisplatin

Expression profile and function of secretogranin V, and its effects on the malignant behavior of esophageal squamous cell carcinoma

Transcriptomic heterogeneity of non-beta islet cells is associated with type 2 diabetes development in mouse models