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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SCGF Monoclonal antibody (60295-1-Ig)

    WB analysis using 60295-1-Ig (same clone as 60295-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60295-1-Ig (SCGF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60295-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using SCGF Monoclonal antibody (60295-1-Ig)

    WB analysis of human heart using 60295-1-Ig (same clone as 60295-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 60295-1-Ig (CLEC11A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using SCGF Monoclonal antibody (60295-1-Ig)

    IHC staining of human lymphoma using 60295-1-Ig (same clone as 60295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60295-1-Ig (CLEC11A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60295-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using SCGF Monoclonal antibody (60295-1-Ig)

    IHC staining of human lymphoma using 60295-1-Ig (same clone as 60295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60295-1-Ig (CLEC11A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60295-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human lymphoma tissue using SCGF Monoclonal antibody (60295-1-Ig)

    IF Staining of human lymphoma using 60295-1-Ig (same clone as 60295-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using SCGF antibody (60295-1-Ig, Clone: 4H11E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60295-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SCGF Monoclonal antibody (60295-1-Ig)

    IF Staining of HepG2 using 60295-1-Ig (same clone as 60295-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SCGF antibody (60295-1-Ig, Clone: 4H11E4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 60295-1-PBS in a different storage buffer formulation.

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SCGF Monoclonal antibody, PBS Only

SCGF Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 60295-1-PBS
Clone No.4H11E4

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

CLEC11A, 4H11E4, LSLCL, C-type lectin superfamily member 3, C-type lectin domain family 11 member A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1mg/ml
100 μg, 1mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60295-1-PBS targets SCGF in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SCGF fusion protein Ag21365 相同性解析による交差性が予測される生物種
Full Name C-type lectin domain family 11, member A
Calculated molecular weight 36 kDa
Observed molecular weight36-40 kDa
GenBank accession numberBC005810
Gene Symbol SCGF
Gene ID (NCBI) 6320
RRIDAB_2881410
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y240
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CLEC11A, also named as CLECSF3, LSLCL, SCGF and p47, stimulates the proliferation and differentiation of hematopoietic precursor cells from various lineages, including erythrocytes, lymphocytes, granulocytes and macrophages. CLEC11A acts synergistically with other cytokines, including IL-3, GCSF, GMCSF and FLT3 ligand. It suppresses SCF-stimulated erythrocyte proliferation.