• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SCMH1 Polyclonal antibody

SCMH1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11489-1-AP

Synonyms

Polycomb protein SCMH1, SCMH1, Scml3, Sex comb on midleg homolog 1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using SCMH1 Polyclonal antibody (11489-1-AP)

    WB analysis using 11489-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11489-1-AP (SCMH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using SCMH1 Polyclonal antibody (11489-1-AP)

    WB analysis of HT-1080 using 11489-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 11489-1-AP (SCMH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SCMH1 Polyclonal antibody (11489-1-AP)

    IHC staining of human pancreas cancer using 11489-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11489-1-AP (SCMH1 antibody) at dilution of 1:100 (under 10x lens).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inHT-1080 cells, U-251 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11489-1-AP targets SCMH1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCMH1 fusion protein Ag1972 相同性解析による交差性が予測される生物種
Full Name sex comb on midleg homolog 1 (Drosophila)
Calculated molecular weight599aa,66 kDa; 660aa,73 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC021252
Gene symbol SCMH1
Gene ID (NCBI) 22955
RRIDAB_2183702
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SCMH1 antibody 11489-1-APDownload protocol
IHC protocol for SCMH1 antibody 11489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols