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Species Reactivity

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  • Featured Product
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SCML1 Polyclonal antibody

SCML1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30257-1-AP

Synonyms

SCML1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SCML1 Polyclonal antibody (30257-1-AP)

    WB analysis using 30257-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30257-1-AP (SCML1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SCML1 Polyclonal antibody (30257-1-AP)

    WB analysis of HepG2 using 30257-1-AP

    WB result of SCML1 antibody (30257-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SCML1 transfected HepG2 cells.

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Tested Applications

Positive WB detected inA549 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30257-1-AP targets SCML1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCML1 fusion protein Ag33003 相同性解析による交差性が予測される生物種
Full Name sex comb on midleg-like 1 (Drosophila)
Calculated molecular weight 37 kDa
Observed molecular weight42 kDa
GenBank accession numberBC026159
Gene symbol SCML1
Gene ID (NCBI) 6322
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sex comb on midleg-like 1 (SCML1) is a meiosis-specific protein and is an essential component of the meiotic dense body. SCML1 is preferentially expressed in germ stem cells of testis, therefore likely involved in spermatogenesis.

Protocols

Product Specific Protocols
WB protocol for SCML1 antibody 30257-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols