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  • Featured Product
  • KD/KO Validated

SCML2 Polyclonal antibody, PBS Only

SCML2 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 25544-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SCML2 Polyclonal antibody (25544-1-AP)

    WB analysis using 25544-1-AP (same clone as 25544-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25544-1-AP (SCML2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25544-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using SCML2 Polyclonal antibody (25544-1-AP)

    WB analysis of HEK-293 using 25544-1-AP (same clone as 25544-1-PBS)

    WB result of SCML2 antibody (25544-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SCML2 transfected HEK-293 cells. This data was developed using the same antibody clone with 25544-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using SCML2 Polyclonal antibody (25544-1-AP)

    WB analysis of mouse testis using 25544-1-AP (same clone as 25544-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25544-1-AP (SCML2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25544-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using SCML2 Polyclonal antibody (25544-1-AP)

    IP experiment of HEK-293 using 25544-1-AP (same clone as 25544-1-PBS)

    IP result of anti-SCML2 (IP:25544-1-AP, 4ug; Detection:25544-1-AP 1:300) with HEK-293 cells lysate 2400ug. This data was developed using the same antibody clone with 25544-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SCML2 Polyclonal antibody (25544-1-AP)

    IF Staining of HEK-293 using 25544-1-AP (same clone as 25544-1-PBS)

    Immunofluorescent analysis of HEK-293 cells using 25544-1-AP (SCML2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25544-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25544-1-PBS targets SCML2 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCML2 fusion protein Ag22271 相同性解析による交差性が予測される生物種
Full Name sex comb on midleg-like 2 (Drosophila)
Calculated molecular weight 700 aa, 77 kDa
Observed molecular weight 70 kDa, 80 kDa
GenBank accession numberBC064617
Gene symbol SCML2
Gene ID (NCBI) 10389
RRIDAB_2880127
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The human SCML2 gene, a mammalian homologue of the Drosophila PcG protein SCM, encodes two protein isoforms: SCML2A that is bound to chromatin and SCML2B that is predominantly nucleoplasmic (PMID: 24358021). Polycomb repressive complex-1 (PRC1) is essential for the epigenetic regulation of gene expression. SCML2 is a Polycomb-group protein that associates with PRC1. SCML2 participates in the epigenetic control of transcription directly and in cooperation with PRC1 (PMID: 24986859).