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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SCN2A Polyclonal antibody (27551-1-AP)

    WB analysis using 27551-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27551-1-AP (SCN2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SCN2A Polyclonal antibody (27551-1-AP)

    IF Staining of mouse brain using 27551-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SCN2A antibody (27551-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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SCN2A Polyclonal antibody

SCN2A Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 27551-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Synonyms

Na(v)1.2, HBSCII, HBSCI, HBSC II, HBA

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27551-1-AP targets SCN2A in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCN2A fusion protein Ag26022 相同性解析による交差性が予測される生物種
Full Name sodium channel, voltage-gated, type II, alpha subunit
Calculated molecular weight 228 kDa
Observed molecular weight~250 kDa
GenBank accession numberNM_001040142
Gene Symbol SCN2A
Gene ID (NCBI) 6326
RRIDAB_3085969
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99250
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Sodium Channel, Voltage-Gated, Type II, Alpha (SCN2A) gene encodes the neuronal sodium channel NaV1.2. There are two major developmentally regulated splice isoforms of NaV1.2 that use mutually exclusive copies of the fifth coding exon and differ by one amino acid at position 209: Asparagine (Asn/N) vs. Aspartic acid (Asp/D). NaV1.2 is one of several sodium channels involved in initiation and propagation of action potentials in neurons. It's widely expressed throughout the human central nervous system, but not in peripheral tissues. (PMID: 29691040)

Protocols

Product Specific Protocols
WB protocol for SCN2A antibody 27551-1-APDownload protocol
IF protocol for SCN2A antibody 27551-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols