Nav1.5 Polyclonal antibody
Nav1.5 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 23016-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 10 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
The immunogen of 23016-1-AP is Nav1.5 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Nav1.5 fusion protein Ag19275 相同性解析による交差性が予測される生物種 |
| Full Name | sodium channel, voltage-gated, type V, alpha subunit |
| Calculated molecular weight | 2016 aa, 227 kDa |
| Observed molecular weight | 227 kDa |
| GenBank accession number | BC140813 |
| Gene symbol | SCN5A |
| Gene ID (NCBI) | 6331 |
| RRID | AB_2879198 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Voltage-gated sodium channels are responsible for initiation and propagation of action potentials in the membranes of neurons and most electrically excitable cells (PMID: 10798388). These channels are composed of a large alpha subunit that forms the ion conduction pore and auxiliary beta subunits (PMID: 11486343). The alpha subunits form a gene family with at least 10 members. Nav1.5, encoded by the SCN5A gene in humans, is a pore forming alpha subunit of voltage-gated sodium channels. Nav1.5 is the principal Na+ channel isoform expressed in cardiomyocytes. Mutations in SCN5A gene have been linked to many cardiac electrical disorders, including the congenital and acquired long QT syndrome, Brugada syndrome, conduction slowing, sick sinus syndrome, atrial fibrillation, and dilated cardiomyopathy (PMID: 23123192).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Nav1.5 antibody 23016-1-AP | Download protocol |
| IHC protocol for Nav1.5 antibody 23016-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Lett Sialyltransferase ST3GAL4 confers osimertinib resistance and offers strategies to overcome resistance in non-small cell lung cancer | ||
Front Pharmacol Spider venom-derived peptide JZTX-14 prevents migration and invasion of breast cancer cells via inhibition of sodium channels | ||
Hum Mol Genet Mice lacking MBNL1 and MBNL2 exhibit sudden cardiac death and molecular signatures recapitulating myotonic dystrophy. | ||
Eur J Pharmacol Glucose fluctuations aggravated the late sodium current induced ventricular arrhythmias via the activation of ROS/CaMKII pathway | ||
Biochim Biophys Acta Mol Basis Dis Changes in ion channel expression and function associated with cardiac arrhythmogenic remodeling by Sorbs2. | ||
J Biol Chem Mechanistic insights into the interaction of the MOG1 protein with the cardiac sodium channel Nav1.5 clarify the molecular basis of Brugada syndrome. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Udesh (Verified Customer) (01-19-2023) | Used at 1:500 dilution in 5% BSA and probed overnight at 4 degrees C. Upper membrane piece shows Nav1.5 bands close to 150 kD. Multiple bands seen probably due to BSA.
 |
FH Rebecca (Verified Customer) (03-16-2022) | 10ug of protein was loaded. Transference at 4ºC and 90V, for 2h. Antibody incubation ON at 4ºC.
 |
