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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SCN5A Monoclonal antibody, PBS Only

SCN5A Monoclonal Antibody for Indirect ELISA, IHC, WB

Host / Isotype

Mouse / IgG1

Reactivity

rabbit, rat, mouse, human

Applications

Indirect ELISA, IHC, WB

Conjugate

Unconjugated

CloneNo.

1C2B3

Cat no : 68273-1-PBS

Synonyms

Nav1.5, HH1, CMPD2, CMD1E, CDCD2

Validation Data Gallery

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  • Western Blot (WB) analysis of rabbit heart tissue using SCN5A Monoclonal antibody (68273-1-Ig)

    WB analysis of rabbit heart using 68273-1-Ig (same clone as 68273-1-PBS)

    rabbit heart tissue were subjected to SDS PAGE followed by western blot with 68273-1-Ig (SCN5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68273-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using SCN5A Monoclonal antibody (68273-1-Ig)

    WB analysis of rat heart using 68273-1-Ig (same clone as 68273-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 68273-1-Ig (SCN5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68273-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using SCN5A Monoclonal antibody (68273-1-Ig)

    IHC staining of mouse skeletal muscle using 68273-1-Ig (same clone as 68273-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68273-1-Ig (SCN5A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68273-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using SCN5A Monoclonal antibody (68273-1-Ig)

    FC experiment of HeLa using 68273-1-Ig (same clone as 68273-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SCN5A (68273-1-Ig, Clone:1C2B3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68273-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68273-1-PBS targets SCN5A in Indirect ELISA, IHC, WB applications and shows reactivity with rabbit, rat, mouse, human samples.

Tested Reactivity rabbit, rat, mouse, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SCN5A fusion protein Ag19275 相同性解析による交差性が予測される生物種
Full Name sodium channel, voltage-gated, type V, alpha subunit
Calculated molecular weight 2016 aa, 227 kDa
Observed molecular weight227 kDa
GenBank accession numberBC140813
Gene symbol SCN5A
Gene ID (NCBI) 6331
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Voltage-gated sodium channels are responsible for initiation and propagation of action potentials in the membranes of neurons and most electrically excitable cells (PMID: 10798388). These channels are composed of a large alpha subunit that forms the ion conduction pore and auxiliary beta subunits (PMID: 11486343). The alpha subunits form a gene family with at least 10 members. Nav1.5, encoded by the SCN5A gene in humans, is a pore forming alpha subunit of voltage-gated sodium channels. Nav1.5 is the principal Na+ channel isoform expressed in cardiomyocytes. Mutations in SCN5A gene have been linked to many cardiac electrical disorders, including the congenital and acquired long QT syndrome, Brugada syndrome, conduction slowing, sick sinus syndrome, atrial fibrillation, and dilated cardiomyopathy (PMID: 23123192).