Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse kidney tissue, rat kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
28707-1-AP targets SCNN1A in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SCNN1A fusion protein Ag30063 相同性解析による交差性が予測される生物種 |
| Full Name | sodium channel, nonvoltage-gated 1 alpha |
| Calculated molecular weight | 76 kDa |
| Observed molecular weight | 60-70 kDa |
| GenBank accession number | BC006526 |
| Gene Symbol | SCNN1A |
| Gene ID (NCBI) | 6337 |
| RRID | AB_2918193 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P37088 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Amiloride-sensitive sodium channel subunit alpha (SCNN1A, also known as Alpha-ENaC and ENaCA) is essential for maintaining sodium homeostasis and is particularly important in the distal nephron and late distal convoluted tubule of the kidney, where aldosterone triggers ENaC activity (PMID: 18086683; 31654198). The ~97 kDa and ~75kDa bands represent the glycosylated and unglycosylated forms of SCNN1A (PMID: 19462466). It also migrated as intact (≈ 75 kDa) and furin-cleaved (≈ 50 kDa) in urinary extracellular vesicles (uEVs) (PMID: 31654198).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SCNN1A antibody 28707-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
PLoS One The developmental programme for genesis of the entire kidney is recapitulated in Wilms tumour. |