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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SCNN1G Polyclonal antibody (30037-1-AP)

    WB analysis using 30037-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30037-1-AP (SCNN1G antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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SCNN1G Polyclonal antibody

SCNN1G Polyclonal Antibody for WB, ELISA
Cat No. 30037-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, pig

Applications

WB, ELISA

Synonyms

Gamma-ENaC, Gamma NaCH, Gamma ENaC, ENaCgamma, ENaCg

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, COLO 320 cells, PC-3 cells, pig kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30037-1-AP targets SCNN1G in WB, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCNN1G fusion protein Ag32176 相同性解析による交差性が予測される生物種
Full Name sodium channel, nonvoltage-gated 1, gamma
Calculated molecular weight 74 kDa
Observed molecular weight74 kDa
GenBank accession numberBC059391
Gene Symbol SCNN1G
Gene ID (NCBI) 6340
RRIDAB_3669702
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51170
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SCNN1G (sodium channel, non-voltage-gated 1, gamma), also known as ENaC gamma (epithelial Na(+) channel subunit gamma) or amiloride-sensitive sodium channel subunit gamma, is the gamma subunit of the epithelial Na(+) channel (ENaC). ENaC is expressed in the apical membrane of salt-absorbing epithelia of the kidney, distal colon, and lung. ENaC is a non-voltage gated, constitutively active channel highly selective for sodium. It has an essential role in salt and fluid homeostasis across epithelial tissues. Mutations in the gene of SCNN1G have been associated with Liddle syndrome. Native SCNN1G has a calculated molecular weight of 74 kDa and may undergo post-transcriptional modifications, including glycosylation and proteolytic cleavage (PMID: 12871941; 18086683).

Protocols

Product Specific Protocols
WB protocol for SCNN1G antibody 30037-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols