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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SCPEP1 Polyclonal antibody (29781-1-AP)

    WB analysis using 29781-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 29781-1-AP (SCPEP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using SCPEP1 Polyclonal antibody (29781-1-AP)

    IHC staining of mouse embryo using 29781-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 29781-1-AP (SCPEP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using SCPEP1 Polyclonal antibody (29781-1-AP)

    IHC staining of mouse embryo using 29781-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 29781-1-AP (SCPEP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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SCPEP1 Polyclonal antibody

SCPEP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29781-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Synonyms

HSCP1, RISC, SCP1, SCPEP1, serine carboxypeptidase 1, UNQ265/PRO302

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells
Positive IHC detected inmouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29781-1-AP targets SCPEP1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCPEP1 fusion protein Ag31396 相同性解析による交差性が予測される生物種
Full Name serine carboxypeptidase 1
Calculated molecular weight 452 aa, 51 kDa
Observed molecular weight33-51 kDa
GenBank accession numberBC072405
Gene Symbol SCPEP1
Gene ID (NCBI) 59342
RRIDAB_2923608
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HB40
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SCPEP1 encodes retinoid-inducible serine carboxypeptidase (PMID:12975309). SCPEP1 and Cathepsin A play roles in regulation of vasoconstriction via inactivation of endothelin-1 (PubMed:24586188). The protein is widely expressed during mouse embryogenesis by immunohistochemical (PubMed:16461364). Scpep1 is proteolytically cleaved to a mature enzyme which is smaller than 51 kDa (PMID:16461364).

Protocols

Product Specific Protocols
WB protocol for SCPEP1 antibody 29781-1-APDownload protocol
IHC protocol for SCPEP1 antibody 29781-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols