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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SCRN3 Polyclonal antibody

SCRN3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13240-1-AP

Synonyms

SES3, Secernin-3, secernin 3

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using SCRN3 Polyclonal antibody (13240-1-AP)

    WB analysis of HepG2 using 13240-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13240-1-AP (SCRN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SCRN3 Polyclonal antibody (13240-1-AP)

    WB analysis of HEK-293 using 13240-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13240-1-AP (SCRN3 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using SCRN3 Polyclonal antibody (13240-1-AP)

    WB analysis of A549 using 13240-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 13240-1-AP (SCRN3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using SCRN3 Polyclonal antibody (13240-1-AP)

    IP experiment of HEK-293 using 13240-1-AP

    IP result of anti-SCRN3 (IP:13240-1-AP, 4ug; Detection:13240-1-AP 1:800) with HEK-293 cells lysate 1760ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SCRN3 Polyclonal antibody (13240-1-AP)

    IHC staining of human kidney using 13240-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13240-1-AP (SCRN3 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using SCRN3 Polyclonal antibody (13240-1-AP)

    IHC staining of human pancreas using 13240-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13240-1-AP (SCRN3 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using SCRN3 Polyclonal antibody (13240-1-AP)

    IHC staining of human pancreas using 13240-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13240-1-AP (SCRN3 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SCRN3 Polyclonal antibody (13240-1-AP)

    IHC staining of human kidney using 13240-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13240-1-AP (SCRN3 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SCRN3 Polyclonal antibody (13240-1-AP)

    IF Staining of HepG2 using 13240-1-AP

    Immunofluorescent analysis of HepG2 cells using 13240-1-AP (SCRN3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, A549 cells, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13240-1-AP targets SCRN3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCRN3 fusion protein Ag3777 相同性解析による交差性が予測される生物種
Full Name secernin 3
Calculated molecular weight 424 aa, 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC031821
Gene symbol SCRN3
Gene ID (NCBI) 79634
RRIDAB_2184816
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SCRN genes encode protein members of the secernin family including Secernin 1, Secernin 2 and Secernin 3. Function of Secernin 2 and Secernin 3 are so far not well understood. Secernin 1 is a novel 50-kDa cytosolic protein that appears to be involved in the regulation of exocytosis from peritoneal mast cells. Secernin-1 a novel tumor-associated antigen (TAA) and may be a universal marker of different cancer types including gastric cancer, which has been validated by several researches. Measurement of SCRN1 can be used in the early detection of cancer or in the surveillance of patients who undergo surgery.

Protocols

Product Specific Protocols
WB protocol for SCRN3 antibody 13240-1-APDownload protocol
IHC protocol for SCRN3 antibody 13240-1-APDownload protocol
IF protocol for SCRN3 antibody 13240-1-APDownload protocol
IP protocol for SCRN3 antibody 13240-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Kwan Ho (Verified Customer) (05-13-2022)

This Ab can detect endogenous SCRN3 in HEK293T cells, which was validated with KO cells. However, it also binds to off-targets.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T cells