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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SCRT1 Polyclonal antibody (31577-1-AP)

    WB analysis using 31577-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31577-1-AP (SCRT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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SCRT1 Polyclonal antibody

SCRT1 Polyclonal Antibody for WB, ELISA
Cat No. 31577-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

SCRT, Scratch 1, hScrt

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inTT cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31577-1-AP targets SCRT1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCRT1 fusion protein Ag36215 相同性解析による交差性が予測される生物種
Full Name scratch homolog 1, zinc finger protein (Drosophila)
Observed molecular weight36 kDa
GenBank accession numberBC168377
Gene Symbol SCRT1
Gene ID (NCBI) 83482
RRIDAB_3670042
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9BWW7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SCRT1 (Transcriptional repressor scratch 1), which is expressed in many tissues, especially in brain and adrenal gland. In addition, SCRT1 is also expressed in islet β cells, and its expression is related to insulin secretion ability. SCRT1 is a C2H2 zinc finger transcription inhibitor, which can bind to E-box motif. The encoded protein may promote nerve differentiation and may be related to cancer with neuroendocrine characteristics. The functions of SCRT1 in islet β cells include regulating the expression and secretion of insulin and influencing the proliferation of β cells. SCRT1 may control calcineurin /NFAT signaling pathway by affecting the expression of Syt4, Notch1, Nfatc1 and Nfatc2 genes, which is an important regulator of the growth and function of β cells (PMID: 33309639). The molecular weight of SCRT1 is 36 kDa.

Protocols

Product Specific Protocols
WB protocol for SCRT1 antibody 31577-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols