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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SCTR Recombinant antibody (83675-6-RR)

    WB analysis using 83675-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83675-6-RR (SCTR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using SCTR Recombinant antibody (83675-6-RR)

    IF Staining of THP-1 using 83675-6-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using SCTR antibody (83675-6-RR, Clone: 240675E6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83675-6-RR

    Affinity and Kinetic Characterization of 83675-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83675-6-RR against Human SCTR were performed. The affinity constant is 6.05 nM.

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SCTR Recombinant antibody

SCTR Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83675-6-RR
Clone No.240675E6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

SR, secretin receptor, SCT-R, SCT R, 240675E6

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTHP-1 cells, mouse pancreas tissue
Positive IF/ICC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83675-6-RR targets SCTR in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SCTR fusion protein Ag5371 相同性解析による交差性が予測される生物種
Full Name SCTR
Calculated molecular weight 440 aa, 50 kDa
Observed molecular weight45-50 kDa
GenBank accession numberBC035757
Gene Symbol SCTR
Gene ID (NCBI) 6344
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP47872
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SCTR is a member of the family B G protein-coupled receptors. SCTR is a receptor for SCT, a gastrointestinal peptide hormone secreted by the S cells of the duodenum. SCT regulates water homeostasis throughout the body and influences the environment of the duodenum by regulating SCT in the stomach and pancreas. Studies suggest that SCT can act as a neuropeptide within the central nervous system (CNS), thus SCTR may regulate the function of the CNS.

Protocols

Product Specific Protocols
WB protocol for SCTR antibody 83675-6-RRDownload protocol
IF protocol for SCTR antibody 83675-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols