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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Anti-Human CD138/Syndecan-1 Rabbit Recombinant Ant (98051-1-RR)

    IHC staining of human tonsillitis using 98051-1-RR (same clone as 98051-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98051-1-RR (CD138/Syndecan-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98051-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Anti-Human CD138/Syndecan-1 Rabbit Recombinant Ant (98051-1-RR)

    IHC staining of human tonsillitis using 98051-1-RR (same clone as 98051-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98051-1-RR (CD138/Syndecan-1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98051-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U266 cells using Anti-Human CD138/Syndecan-1 Rabbit Recombinant Ant (98051-1-PBS)

    FC experiment of U266 using 98051-1-PBS

    1x10^6 U266 cells (left) or Jurkat cells (right) were surface stained with 0.25 ug Anti-Human CD138/Syndecan-1 Rabbit Recombinant Antibody, PBS Only (98051-1-PBS, Clone: 240422C4) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Flow cytometry (FC) experiment of U266 cells using Anti-Human CD138/Syndecan-1 Rabbit Recombinant Ant (98051-1-RR)

    FC experiment of U266 using 98051-1-RR (same clone as 98051-1-PBS)

    1x10^6 U266 cells (left) or Jurkat cells (right) were surface stained with 0.25 ug Anti-Human CD138/Syndecan-1 Rabbit Recombinant Antibody (98051-1-RR, Clone: 240422C4) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (blue). Cells were not fixed. This data was developed using the same antibody clone with 98051-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98051-1-RR

    Affinity and Kinetic Characterization of 98051-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98051-1-RR against Human CD138/Syndecan-1 were performed. The affinity constant is 20.8 nM.

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Anti-Human CD138/Syndecan-1 Rabbit Recombinant Antibody, PBS Only

CD138/Syndecan-1 Uni-rAbTM Recombinant Antibody for IHC, FC
Cat No. 98051-1-PBS
Clone No.240422C4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, FC

Synonyms

CD138, 240422C4, syndecan, SYND1, SDC1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive FC detected inU266 cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98051-1-PBS targets CD138/Syndecan-1 in IHC, FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name syndecan 1
Calculated molecular weight 32.5 kDa
GenBank accession numberBC008765
Gene Symbol CD138
Gene ID (NCBI) 6382
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP18827
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD138, also named as Syndecan-1 (SDC1), is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. CD138 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas.

Protocols

Product Specific Protocols
FC protocol for CD138/Syndecan-1 antibody 98051-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols